Differences in Transcriptional Dynamics Between T-cells and Macrophages as Determined by a Three-State Mathematical Model.
Anti-HIV Agents
/ pharmacology
Cell Line
Drug Resistance, Viral
/ drug effects
Gene Expression Regulation, Viral
/ immunology
HIV Infections
/ blood
HIV Long Terminal Repeat
/ genetics
HIV-1
/ drug effects
Humans
Macrophages
/ immunology
Models, Genetic
Models, Immunological
Primary Cell Culture
RNA, Viral
/ genetics
T-Lymphocytes
/ immunology
Transcription, Genetic
/ drug effects
Virus Replication
/ drug effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 02 2020
10 02 2020
Historique:
received:
18
10
2018
accepted:
17
01
2020
entrez:
12
2
2020
pubmed:
12
2
2020
medline:
20
11
2020
Statut:
epublish
Résumé
HIV-1 viral transcription persists in patients despite antiretroviral treatment, potentially due to intermittent HIV-1 LTR activation. While several mathematical models have been explored in the context of LTR-protein interactions, in this work for the first time HIV-1 LTR model featuring repressed, intermediate, and activated LTR states is integrated with generation of long (env) and short (TAR) RNAs and proteins (Tat, Pr55, and p24) in T-cells and macrophages using both cell lines and infected primary cells. This type of extended modeling framework allows us to compare and contrast behavior of these two cell types. We demonstrate that they exhibit unique LTR dynamics, which ultimately results in differences in the magnitude of viral products generated. One of the distinctive features of this work is that it relies on experimental data in reaction rate computations. Two RNA transcription rates from the activated promoter states are fit by comparison of experimental data to model predictions. Fitting to the data also provides estimates for the degradation/exit rates for long and short viral RNA. Our experimentally generated data is in reasonable agreement for the T-cell as well macrophage population and gives strong evidence in support of using the proposed integrated modeling paradigm. Sensitivity analysis performed using Latin hypercube sampling method confirms robustness of the model with respect to small parameter perturbations. Finally, incorporation of a transcription inhibitor (F07#13) into the governing equations demonstrates how the model can be used to assess drug efficacy. Collectively, our model indicates transcriptional differences between latently HIV-1 infected T-cells and macrophages and provides a novel platform to study various transcriptional dynamics leading to latency or activation in numerous cell types and physiological conditions.
Identifiants
pubmed: 32042107
doi: 10.1038/s41598-020-59008-0
pii: 10.1038/s41598-020-59008-0
pmc: PMC7010665
doi:
Substances chimiques
Anti-HIV Agents
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2227Références
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