Cytotoxic and proapoptotic effect of doxycycline - An in vitro study on the human skin melanoma cells.
Apoptosis
Cell cycle
DNA fragmentation
Doxycycline
Melanoma
Mitochondrial membrane potential
Journal
Toxicology in vitro : an international journal published in association with BIBRA
ISSN: 1879-3177
Titre abrégé: Toxicol In Vitro
Pays: England
ID NLM: 8712158
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
26
11
2019
revised:
04
02
2020
accepted:
06
02
2020
pubmed:
12
2
2020
medline:
5
1
2021
entrez:
12
2
2020
Statut:
ppublish
Résumé
Doxycycline is a semisynthetic, second generation tetracycline. Currently, it is used, among others, in the treatment of acne and skin infections. Moreover, doxycycline has many valuable nonantibiotic properties, including anti-inflammatory, immunosuppressive and anticancer effects. Recent studies showed that the drug had the ability to inhibit the adhesion and migration of cancer cells, as well as affected their growth and proliferation and induced apoptosis. The purpose of this study was to examine the antimelanoma effect of doxycycline. The obtained results demonstrated that doxycycline decreased the viability and inhibited the proliferation of human melanoma cells, proportionally to the drug concentration and the treatment time. It was stated that doxycycline disturbed the homeostasis of the cells by lowering intracellular level of reduced thiols. In addition, the treatment changed the cell cycle profile and triggered the DNA fragmentation. Mitochondria of melanoma cells exposed to the drug had lowered membrane potential, which indicated cells apoptosis. Finally, doxycycline induced the externalization phosphatidylserine - a well-known hallmark of apoptosis, confirmed by results of annexin V test. The presented study contributes to the increase of knowledge about nonantibacterial action of doxycycline, including the influence on human cancer cells and indicates new potential possibility of effective treatment of malignant melanoma.
Identifiants
pubmed: 32044399
pii: S0887-2333(19)30894-X
doi: 10.1016/j.tiv.2020.104790
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
Doxycycline
N12000U13O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104790Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors: Jakub Rok, Marta Karkoszka, Zuzanna Rzepka, Michalina Respondek, Klaudia Banach, Artur Beberok and Dorota Wrześniok declare that there are no conflicts of interest. The manuscript entitled: “Cytotoxic and proapoptotic effect of doxycycline – an in vitro study on the human skin melanoma cells”, which all the listed authors have read and approved of, has not been submitted elsewhere for publication, in whole or in part, and is permitted to be published in this journal.