The extracellular and intracellular regions of Crb2a play distinct roles in guiding the formation of the apical zonula adherens.
Adherens junctions
Crumbs
Outer limiting membrane
Punctum adherens
Zonula adherens
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
01
11
2019
revised:
18
01
2020
accepted:
23
01
2020
pubmed:
12
2
2020
medline:
5
1
2021
entrez:
12
2
2020
Statut:
ppublish
Résumé
The transmembrane protein Crumbs (Crb), a key regulator of apical polarity, has a known involvement in establishment of the apical zonula adherens in epithelia, although the precise mechanism remains elusive. The zonula adherens are required to maintain the integrity and orderly arrangement of epithelia. Loss of the zonula adherens leads to morphogenetic defects in the tissues derived from epithelium. In this study, we revealed that the intracellular tail of Crb2a promoted the apical distribution of adherens junctions (AJs) in zebrafish retinal and lens epithelia, but caused assembly into unstable punctum adherens-like adhesion plaques. The extracellular region of Crb2a guided the transformation of AJs from the punctum adherens into stable zonula adherens. Accordingly, a truncated form of Crb2a lacking the extracellular region (Crb2a
Identifiants
pubmed: 32044715
pii: S0753-3322(20)30132-3
doi: 10.1016/j.biopha.2020.109942
pii:
doi:
Substances chimiques
Crb2a protein, zebrafish
0
Membrane Proteins
0
Zebrafish Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109942Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no competing or financial interests