Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay.

African Swine Fever Virus / genetics Animals CRISPR-Cas Systems / genetics Clustered Regularly Interspaced Short Palindromic Repeats / genetics Listeria monocytogenes / genetics Nucleic Acids / genetics Real-Time Polymerase Chain Reaction Swine
ASFV detection CRISPR/Cas9 GMOs detection Listeria monocytogenes detection lateral flow assay

Journal

ACS nano
ISSN: 1936-086X
Titre abrégé: ACS Nano
Pays: United States
ID NLM: 101313589

Informations de publication

Date de publication:
25 02 2020
Historique:
pubmed: 12 2 2020
medline: 19 12 2020
entrez: 12 2 2020
Statut: ppublish

Résumé

The lateral flow assay is one of the most convenient analytical techniques for analyzing the immune response, but its applicability to precise genetic analyses is limited by the false-positive signal and tedious and inefficient hybridization steps. Here, we introduce the CRISPR (clustered regularly interspaced short palindromic repeats) /Cas system into the lateral flow assay, termed CRISPR/Cas9-mediated lateral flow nucleic acid assay (CASLFA), to address such issues. In this study, CASLFA is utilized to identify

Identifiants

DOI: 10.1021/acsnano.0c00022 PMID: 32045522
pubmed: 32045522
doi: 10.1021/acsnano.0c00022
doi:

Substances chimiques

Nucleic Acids 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2497-2508

Auteurs

Xusheng Wang (X)

College of Biophotonics & School of Life Sciences , South China Normal University , Guangzhou 510631 , China.

Erhu Xiong (E)

College of Biophotonics & School of Life Sciences , South China Normal University , Guangzhou 510631 , China.

Tian Tian (T)

College of Biophotonics & School of Life Sciences , South China Normal University , Guangzhou 510631 , China.

Meng Cheng (M)

College of Biophotonics & School of Life Sciences , South China Normal University , Guangzhou 510631 , China.

Wei Lin (W)

College of Biophotonics & School of Life Sciences , South China Normal University , Guangzhou 510631 , China.

Heng Wang (H)

Guangdong Laboratory for Lingnan Modern Agriculture , Guangzhou 510642 , China.
Research Center for African Swine Fever Prevention and Control , South China Agricultural University , Guangzhou 510642 , China.

Guihong Zhang (G)

Guangdong Laboratory for Lingnan Modern Agriculture , Guangzhou 510642 , China.
National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria , South China Agricultural University , Guangzhou 510642 , China.
Research Center for African Swine Fever Prevention and Control , South China Agricultural University , Guangzhou 510642 , China.

Jian Sun (J)

Guangdong Laboratory for Lingnan Modern Agriculture , Guangzhou 510642 , China.
National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria , South China Agricultural University , Guangzhou 510642 , China.
Research Center for African Swine Fever Prevention and Control , South China Agricultural University , Guangzhou 510642 , China.

Xiaoming Zhou (X)

College of Biophotonics & School of Life Sciences , South China Normal University , Guangzhou 510631 , China.
ORCID: 0000-0001-5597-4804

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Classifications MeSH

questionsmedicales.fr › Virus › Virus à ADN › Asfarviridae › Virus de la peste porcine africaine › Clustered Regularly Interspaced Short...
questionsmedicales.fr › Eucaryotes › Animaux › Clustered Regularly Interspaced Short...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Clustered Regularly Interspaced Short...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › Clustered Regularly Interspaced Short...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Listeria › Listeria monocytogenes › Clustered Regularly Interspaced Short...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › Clustered Regularly Interspaced Short...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Clustered Regularly Interspaced Short...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Clustered Regularly Interspaced Short...