Methylation of geometrically constrained lysine analogues by histone lysine methyltransferases.


Journal

Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838

Informations de publication

Date de publication:
10 Mar 2020
Historique:
pubmed: 13 2 2020
medline: 25 3 2020
entrez: 13 2 2020
Statut: ppublish

Résumé

We report synthesis and enzymatic assays on human histone lysine methyltransferase catalysed methylation of histones that possess lysine and its geometrically constrained analogues containing rigid (E)-alkene (KE), (Z)-alkene (KZ) and alkyne (Kyne) moieties. Methyltransferases G9a and GLP do have a capacity to catalyse methylation in the order K ≫ KE > KZ ∼ Kyne, whereas monomethyltransferase SETD8 catalyses only methylation of K and KE.

Identifiants

pubmed: 32048637
doi: 10.1039/c9cc09098c
doi:

Substances chimiques

Alkenes 0
Alkynes 0
Histone-Lysine N-Methyltransferase EC 2.1.1.43
Lysine K3Z4F929H6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3039-3042

Auteurs

Abbas H K Al Temimi (AHK)

Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. Floris.Rutjes@ru.nl.

Paul B White (PB)

Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. Floris.Rutjes@ru.nl.

Marcus J M Mulders (MJM)

Mercachem B.V., Kerkenbos 1013, 6546 BB Nijmegen, The Netherlands. anita.wegert@mercachem.nl.

Nicole G A van der Linden (NGA)

Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. Floris.Rutjes@ru.nl.

Richard H Blaauw (RH)

Chiralix B.V., Kerkenbos 1013, 6546 BB Nijmegen, The Netherlands.

Anita Wegert (A)

Mercachem B.V., Kerkenbos 1013, 6546 BB Nijmegen, The Netherlands. anita.wegert@mercachem.nl.

Floris P J T Rutjes (FPJT)

Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. Floris.Rutjes@ru.nl.

Jasmin Mecinović (J)

Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. Floris.Rutjes@ru.nl and University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark. mecinovic@sdu.dk.

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Classifications MeSH