Cellular and Molecular Probing of Intact Human Organs.
Aged, 80 and over
Animals
Brain
/ diagnostic imaging
Deep Learning
Eye
/ diagnostic imaging
Female
Humans
Imaging, Three-Dimensional
/ methods
Kidney
/ diagnostic imaging
Limit of Detection
Male
Mice
Middle Aged
Optical Imaging
/ methods
Pancreas
/ diagnostic imaging
Staining and Labeling
/ methods
Swine
Thyroid Gland
/ diagnostic imaging
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
20 02 2020
20 02 2020
Historique:
received:
22
05
2019
revised:
04
12
2019
accepted:
22
01
2020
pubmed:
16
2
2020
medline:
25
8
2020
entrez:
16
2
2020
Statut:
ppublish
Résumé
Optical tissue transparency permits scalable cellular and molecular investigation of complex tissues in 3D. Adult human organs are particularly challenging to render transparent because of the accumulation of dense and sturdy molecules in decades-aged tissues. To overcome these challenges, we developed SHANEL, a method based on a new tissue permeabilization approach to clear and label stiff human organs. We used SHANEL to render the intact adult human brain and kidney transparent and perform 3D histology with antibodies and dyes in centimeters-depth. Thereby, we revealed structural details of the intact human eye, human thyroid, human kidney, and transgenic pig pancreas at the cellular resolution. Furthermore, we developed a deep learning pipeline to analyze millions of cells in cleared human brain tissues within hours with standard lab computers. Overall, SHANEL is a robust and unbiased technology to chart the cellular and molecular architecture of large intact mammalian organs.
Identifiants
pubmed: 32059778
pii: S0092-8674(20)30111-2
doi: 10.1016/j.cell.2020.01.030
pmc: PMC7557154
mid: NIHMS1628797
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
796-812.e19Subventions
Organisme : NIA NIH HHS
ID : RF1 AG057575
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests A.E. has filed a patent on SHANEL technologies described in this study.
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