Association of MTHFR C677T polymorphism with elevated homocysteine level and disease development in vitiligo.
Adolescent
Adult
Female
Genetic Association Studies
Genetic Predisposition to Disease
Genotype
Homocysteine
/ blood
Homozygote
Humans
Iran
/ epidemiology
Male
Methylenetetrahydrofolate Reductase (NADPH2)
/ genetics
Middle Aged
Polymorphism, Single Nucleotide
/ genetics
Risk Factors
Vitamin B 12
/ blood
Vitiligo
/ blood
Young Adult
MTHFR C677T polymorphism
homocysteine
vitamin B12
vitiligo
Journal
International journal of immunogenetics
ISSN: 1744-313X
Titre abrégé: Int J Immunogenet
Pays: England
ID NLM: 101232337
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
22
09
2019
revised:
07
01
2020
accepted:
10
01
2020
pubmed:
18
2
2020
medline:
10
6
2021
entrez:
18
2
2020
Statut:
ppublish
Résumé
Increasing evidence on the association of MTHFR gene polymorphism and serum homocysteine levels with autoimmune diseases such as vitiligo has made the MTHFR gene a very interesting candidate to be evaluated in different ethnicities and populations. We aimed to evaluate the levels of serum homocysteine and vitamin B12 and their associations with MTHFR C677T polymorphism in the Iranian population. This case-control study included 104 patients with vitiligo and 100 age- and sex-matched healthy control subjects. Serum vitamin B12 and homocysteine levels were measured by a chemiluminescence assay. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used for genotyping the polymorphism. The mean serum homocysteine levels were significantly higher in cases than controls and associated with disease activity (p < .001). Furthermore, the homozygous MTHFR C677T variant genotype was associated with vitiligo development (adjusted OR: 3.52, 95% CI: 1.09-11.32, p = .02) and elevated homocysteine level (p < .001). There was no association between serum vitamin B12 levels and the MTHFR C677T genotype. The homozygous variant MTHFR C677T may be considered as a risk factor for both elevated homocysteine levels and the development of vitiligo in the Iranian population. Although these results are not conclusive, they could elucidate the contribution of genetic and immune-mediated inflammatory factors to the pathogenesis of vitiligo.
Substances chimiques
Homocysteine
0LVT1QZ0BA
MTHFR protein, human
EC 1.5.1.20
Methylenetetrahydrofolate Reductase (NADPH2)
EC 1.5.1.20
Vitamin B 12
P6YC3EG204
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
342-350Informations de copyright
© 2020 John Wiley & Sons Ltd.
Références
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