Carbohydrate-Binding Capability and Functional Conformational Changes of AbnE, an Arabino-oligosaccharide Binding Protein.

Arabinose / chemistry Bacterial Proteins / chemistry Crystallography, X-Ray Geobacillus stearothermophilus / enzymology Models, Molecular Mutagenesis, Site-Directed Mutation Protein Binding Protein Conformation
ABC transporter Arabinan utilization system Geobacillus stearothermophilus arabinose substrate-binding protein

Journal

Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R

Informations de publication

Date de publication:
27 03 2020
Historique:
received: 10 10 2019
revised: 19 01 2020
accepted: 30 01 2020
pubmed: 19 2 2020
medline: 28 8 2020
entrez: 19 2 2020
Statut: ppublish

Résumé

ABC importers are membrane proteins responsible for the transport of nutrients into the cells of prokaryotes. Although the structures of ABC importers vary, all contain four conserved domains: two nucleotide-binding domains (NBDs), which bind and hydrolyze ATP, and two transmembrane domains (TMDs), which help translocate the substrate. ABC importers are also dependent on an additional protein component, a high-affinity substrate-binding protein (SBP) that specifically binds the target ligand for delivery to the appropriate ABC transporter. AbnE is a SBP belonging to the ABC importer for arabino-oligosaccharides in the Gram-positive thermophilic bacterium Geobacillus stearothermophilus. Using isothermal titration calorimetry (ITC), purified AbnE was shown to bind medium-sized arabino-oligosaccharides, in the range of arabino-triose (A3) to arabino-octaose (A8), all with K

Identifiants

DOI: 10.1016/j.jmb.2020.01.041 PMID: 32067952
pubmed: 32067952
pii: S0022-2836(20)30107-8
doi: 10.1016/j.jmb.2020.01.041
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Arabinose B40ROO395Z

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2099-2120

Informations de copyright

Copyright © 2020. Published by Elsevier Ltd.

Auteurs

Shifra Lansky (S)

Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.

Rachel Salama (R)

Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel.

Smadar Shulami (S)

Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel.

Noa Lavid (N)

Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel.

Saumik Sen (S)

Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel; Fritz Haber Center for Molecular Dynamics Research, Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.

Igor Schapiro (I)

Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel; Fritz Haber Center for Molecular Dynamics Research, Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.

Yuval Shoham (Y)

Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel. Electronic address: yshoham@technion.ac.il.

Gil Shoham (G)

Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel. Electronic address: gil2@vms.huji.ac.il.

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Classifications MeSH

questionsmedicales.fr › Glucides › Sucres › Oses › Pentoses › Arabinose › Carbohydrate-Binding Capability and Functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Carbohydrate-Binding Capability and Functional...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Carbohydrate-Binding Capability and Functional...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Geobacillus › Geobacillus stearothermophilus › Carbohydrate-Binding Capability and Functional...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Carbohydrate-Binding Capability and Functional...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Mutagenèse dirigée › Carbohydrate-Binding Capability and Functional...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Carbohydrate-Binding Capability and Functional...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Carbohydrate-Binding Capability and Functional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Carbohydrate-Binding Capability and Functional...