Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse.
FAHD1
SIRT3
crystallography
Journal
Bioscience reports
ISSN: 1573-4935
Titre abrégé: Biosci Rep
Pays: England
ID NLM: 8102797
Informations de publication
Date de publication:
27 03 2020
27 03 2020
Historique:
received:
23
12
2019
revised:
14
02
2020
accepted:
17
02
2020
pubmed:
19
2
2020
medline:
26
3
2021
entrez:
19
2
2020
Statut:
ppublish
Résumé
FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite high structural similarity with human FAHD1, a rabbit monoclonal antibody (RabMab) could be produced that is able to recognize mouse FAHD1, but not the human form, whereas a polyclonal antibody recognized both proteins. Epitope mapping in combination with our deposited crystal structures revealed that the epitope overlaps with a reported SIRT3 deacetylation site in mouse FAHD1.
Identifiants
pubmed: 32068790
pii: 222164
doi: 10.1042/BSR20194431
pmc: PMC7056447
pii:
doi:
Substances chimiques
Acetoacetates
0
Mitochondrial Proteins
0
fumarylacetoacetate
28613-33-4
FAHD1 protein, human
EC 3.-
Fahd2a protein, mouse
EC 3.-
Hydrolases
EC 3.-
Carboxy-Lyases
EC 4.1.1.-
oxaloacetate decarboxylase
EC 4.1.1.112
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Austrian Science Fund FWF
ID : P 28395
Pays : Austria
Organisme : Austrian Science Fund FWF
ID : P 31582
Pays : Austria
Informations de copyright
© 2020 The Author(s).
Références
Science. 2012 May 25;336(6084):1030-3
pubmed: 22628654
J Appl Crystallogr. 2013 Jun 1;46(Pt 3):804-810
pubmed: 23682196
Cell Metab. 2015 Apr 7;21(4):637-46
pubmed: 25863253
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Acta Crystallogr D Struct Biol. 2016 Apr;72(Pt 4):454-66
pubmed: 27050125
J Biol Chem. 2011 Oct 21;286(42):36500-8
pubmed: 21878618
Mol Cell. 2011 Jan 21;41(2):139-49
pubmed: 21255725
Acta Crystallogr D Biol Crystallogr. 2011 Oct;67(Pt 10):902-6
pubmed: 21931222
J Biol Chem. 2015 Mar 13;290(11):6755-62
pubmed: 25575590
Acta Crystallogr D Struct Biol. 2018 May 1;74(Pt 5):433-440
pubmed: 29717714
Acta Crystallogr D Struct Biol. 2016 Aug;72(Pt 8):966-75
pubmed: 27487827
Exp Gerontol. 2017 Jun;92:7-12
pubmed: 28286170
J Vis Exp. 2019 Jun 20;(148):
pubmed: 31282888
Biochem J. 2018 Nov 20;475(22):3561-3576
pubmed: 30348641
Nucleic Acids Res. 2016 Jan 4;44(D1):D710-6
pubmed: 26687719
Biochem Soc Trans. 2018 Apr 17;46(2):295-309
pubmed: 29487229
Nucleic Acids Res. 2018 Mar 16;46(5):2699
pubmed: 29425356
J Synchrotron Radiat. 2015 Nov;22(6):1540-7
pubmed: 26524320
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
pubmed: 15299926
Nucleic Acids Res. 2014 Jan;42(Database issue):D285-91
pubmed: 24288376
Mech Ageing Dev. 2019 Jan;177:22-29
pubmed: 30055189
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Acta Crystallogr D Biol Crystallogr. 2015 Aug;71(Pt 8):1757-67
pubmed: 26249356
Diabetologia. 2013 May;56(5):1068-77
pubmed: 23397292