Plasmin-mediated proteolysis of human factor IXa in the presence of calcium/phospholipid: Conversion of procoagulant factor IXa to a fibrinolytic enhancer.

Calcium / metabolism Factor IXa / metabolism Fibrinolysin / metabolism Humans Phospholipids Proteolysis Tissue Plasminogen Activator / metabolism
factor IXa far-western blot mass spectrometry proteolysis tissue plasminogen activator

Journal

Journal of thrombosis and haemostasis : JTH
ISSN: 1538-7836
Titre abrégé: J Thromb Haemost
Pays: England
ID NLM: 101170508

Informations de publication

Date de publication:
05 2020
Historique:
received: 15 10 2019
revised: 28 01 2020
accepted: 18 02 2020
pubmed: 20 2 2020
medline: 15 5 2021
entrez: 20 2 2020
Statut: ppublish

Résumé

Factor (F) IX/IXa inactivation by plasmin has been studied; however, whether plasmin converts FIXa to a fibrinolytic enhancer is not known. Investigate plasmin proteolysis site(s) in FIXa that inactivates and transforms it into a fibrinolytic enhancer. NH Plasmin in the presence of Ca Plasmin cleavage at Lys316↓Gly317 abrogates FIXaβ coagulant activity, whereas additional cleavage at Lys413↓Leu414 converts it into a fibrinolytic enhancer.

Sections du résumé

BACKGROUND
Factor (F) IX/IXa inactivation by plasmin has been studied; however, whether plasmin converts FIXa to a fibrinolytic enhancer is not known.
OBJECTIVE
Investigate plasmin proteolysis site(s) in FIXa that inactivates and transforms it into a fibrinolytic enhancer.
METHODS
NH
RESULTS
Plasmin in the presence of Ca
CONCLUSION
Plasmin cleavage at Lys316↓Gly317 abrogates FIXaβ coagulant activity, whereas additional cleavage at Lys413↓Leu414 converts it into a fibrinolytic enhancer.

Identifiants

DOI: 10.1111/jth.14773 PMID: 32073726 PMC: PMC7363452
pubmed: 32073726
doi: 10.1111/jth.14773
pmc: PMC7363452
mid: NIHMS1572064
pii: S1538-7836(22)00319-1
doi:

Substances chimiques

Phospholipids 0
Factor IXa EC 3.4.21.22
Tissue Plasminogen Activator EC 3.4.21.68
Fibrinolysin EC 3.4.21.7
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1171-1182

Subventions

Organisme : NHLBI NIH HHS
ID : R01 HL141850
Pays : United States

Informations de copyright

© 2020 International Society on Thrombosis and Haemostasis.

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Auteurs

Amy E Schmidt (AE)

Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, California.

Kanagasabai Vadivel (K)

Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, California.

Julian Whitelegge (J)

The Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California.

Satya Paul Bajaj (SP)

Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, California.
Molecular Biology Institute, University of California, Los Angeles, California.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Calcium › Plasmin-mediated proteolysis of human factor...
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Facteur IX › Facteur IXa › Plasmin-mediated proteolysis of human factor...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Protéases à sérine › Serine endopeptidases › Fibrinolysine › Plasmin-mediated proteolysis of human factor...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Plasmin-mediated proteolysis of human factor...
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Plasmin-mediated proteolysis of human factor...
questionsmedicales.fr › Métabolisme › Protéolyse › Plasmin-mediated proteolysis of human factor...
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Activateur tissulaire du plasminogène › Plasmin-mediated proteolysis of human factor...