Identification of oxidant susceptible proteins in Salmonella Typhimurium.
HOCl
Oxidative stress
Protein carbonylation
Salmonella
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
29
10
2019
accepted:
13
02
2020
pubmed:
23
2
2020
medline:
21
10
2020
entrez:
21
2
2020
Statut:
ppublish
Résumé
The human gut pathogen, Salmonella Typhimurium (S. Typhimurium) not only survives but also replicates inside the phagocytic cells. Bacterial proteins are the primary targets of phagocyte generated oxidants. Because of the different amino acid composition, some proteins are more prone to oxidation than others. Many oxidant induced modifications to amino acids have been described. Introduction of carbonyl group is one of such modifications, which takes place quite early following exposure of proteins to oxidants and is quite stable. Therefore, carbonyl groups can be exploited to identify oxidant susceptible proteins. Hypochlorous acid (HOCl) is one of the most potent oxidants produced by phagocytes. Incubation of S. Typhimurium with 3 mM HOCl resulted in more than 150 folds loss of bacterial viability. Proteins extracted from HOCl exposed S. Typhimurium cells showed about 60 folds (p < 0.001) more carbonyl levels as compared to unexposed cells. Similarly, 2, 4-Dinitrophenylhydrazine (2, 4-DNPH) derivatized proteins of HOCl treated S. Typhimurium cultures reacted strongly with anti-DNP antibodies as compared to buffer treated counterpart. Next, we have derivatized carbonyl groups on the proteins with biotin hydrazide. The derivatized proteins were then isolated by avidin affinity chromatography. Mass spectrometry based analysis revealed the presence of 204 proteins.
Identifiants
pubmed: 32076998
doi: 10.1007/s11033-020-05328-3
pii: 10.1007/s11033-020-05328-3
doi:
Substances chimiques
Bacterial Proteins
0
Oxidants
0
Hypochlorous Acid
712K4CDC10
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2231-2242Subventions
Organisme : Department of Biotechnology , Ministry of Science and Technology
ID : BT/PR13689/BRB/10/1399/2015
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