In-vivo vascular application via ultra-fast bioprinting for future 5D personalised nanomedicine.
Angioplasty, Balloon
Animals
Arteries
/ diagnostic imaging
Bioprinting
/ methods
Cell Survival
Drug-Eluting Stents
Human Umbilical Vein Endothelial Cells
/ cytology
Humans
Image Processing, Computer-Assisted
Mice
Muscle, Smooth, Vascular
/ cytology
Nanomedicine
/ methods
Nanoparticles
/ chemistry
Percutaneous Coronary Intervention
Porosity
Precision Medicine
Printing, Three-Dimensional
Rats
Rats, Sprague-Dawley
Tomography, X-Ray Computed
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 02 2020
21 02 2020
Historique:
received:
26
09
2019
accepted:
04
02
2020
entrez:
22
2
2020
pubmed:
23
2
2020
medline:
31
12
2020
Statut:
epublish
Résumé
The design of 3D complex structures enables new correlation studies between the engineering parameters and the biological activity. Moreover, additive manufacturing technology could revolutionise the personalised medical pre-operative management due to its possibility to interplay with computer tomography. Here we present a method based on rapid freeze prototyping (RFP) 3D printer, reconstruction cutting, nano dry formulation, fast freeze gelation, disinfection and partial processes for the 5D digital models functionalisation. We elaborated the high-resolution computer tomography scan derived from a complex human peripheral artery and we reconstructed the 3D model of the vessel in order to obtain and verify the additive manufacturing processes. Then, based on the drug-eluting balloon selected for the percutaneous intervention, we reconstructed the biocompatible eluting-freeform coating containing 40 nm fluorescent nanoparticles (NPs) by means of RFP printer and we tested the in-vivo feasibility. We introduced the NPs-loaded 5D device in a rat's vena cava. The coating dissolved in a few minutes releasing NPs which were rapidly absorbed in vascular smooth muscle cell (VSMC) and human umbilical vein endothelial cell (HUVEC) in-vitro. We developed 5D high-resolution self-dissolving devices incorporating NPs with the perspective to apply this method to the personalised medicine.
Identifiants
pubmed: 32081937
doi: 10.1038/s41598-020-60196-y
pii: 10.1038/s41598-020-60196-y
pmc: PMC7035336
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3205Commentaires et corrections
Type : ErratumIn
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