New Developments in Transcriptomic Analysis of Synovial Tissue.
fibroblast
microarray
pathotype
sequencing
single-cell
stratification
synovium
transcriptomics
Journal
Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047
Informations de publication
Date de publication:
2020
2020
Historique:
received:
26
11
2019
accepted:
14
01
2020
entrez:
22
2
2020
pubmed:
23
2
2020
medline:
23
2
2020
Statut:
epublish
Résumé
Transcriptomic technologies are constantly changing and improving, resulting in an ever increasing understanding of gene expression in health and disease. These technologies have been used to investigate the pathological changes occurring in the joints of rheumatoid arthritis patients, leading to discoveries of disease mechanisms, and novel potential therapeutic targets. Microarrays were initially used on both whole tissue and cell subsets to investigate research questions, with bulk RNA sequencing allowing for further elaboration of these findings. A key example is the classification of pathotypes in rheumatoid arthritis using RNA sequencing that had previously been discovered using microarray and histology. Single-cell sequencing has now delivered a step change in understanding of the diversity and function of subpopulations of cells, in particular synovial fibroblasts. Future technologies, such as high resolution spatial transcriptomics, will enable step changes integrating single cell transcriptomic and geographic data to provide an integrated understanding of synovial pathology.
Identifiants
pubmed: 32083090
doi: 10.3389/fmed.2020.00021
pmc: PMC7005068
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
21Subventions
Organisme : Medical Research Council
ID : MC_PC_16037
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M007669/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P021220/1
Pays : United Kingdom
Informations de copyright
Copyright © 2020 Carr, Turner, Major, Scheel-Toellner and Filer.
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