Cellular mRNA export factor UAP56 recognizes nucleic acid binding site of influenza virus NP protein.
DEAD-Box ATPase
Host-pathogen interaction
Influenza A virus
RNA-Binding protein
mRNA nuclear export
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
30 04 2020
30 04 2020
Historique:
received:
03
01
2020
accepted:
09
02
2020
pubmed:
23
2
2020
medline:
11
11
2020
entrez:
23
2
2020
Statut:
ppublish
Résumé
Influenza A virus nucleoprotein (NP) is a structural component that encapsulates the viral genome into the form of ribonucleoprotein complexes (vRNPs). Efficient assembly of vRNPs is critical for the virus life cycle. The assembly route from RNA-free NP to the NP-RNA polymer in vRNPs has been suggested to require a cellular factor UAP56, but the mechanism is poorly understood. Here, we characterized the interaction between NP and UAP56 using recombinant proteins and showed that UAP56 features two NP binding sites. In addition to the UAP56 core comprised of two RecA domains, we identified the N-terminal extension (NTE) of UAP56 as a previously unknown NP binding site. In particular, UAP56-NTE recognizes the nucleic acid binding region of NP. This corroborates our observation that binding of UAP56-NTE and RNA to NP is mutually exclusive. Collectively, our results reveal the molecular basis for how UAP56 acts on RNA-free NP, and provide new insights into NP-mediated influenza genome packaging.
Identifiants
pubmed: 32085897
pii: S0006-291X(20)30327-2
doi: 10.1016/j.bbrc.2020.02.059
pmc: PMC7132624
mid: NIHMS1564198
pii:
doi:
Substances chimiques
NP protein, Influenza A virus
0
Nucleocapsid Proteins
0
RNA, Messenger
0
RNA-Binding Proteins
0
Ribonucleoproteins
0
Viral Core Proteins
0
DDX39B protein, human
EC 3.6.1.-
DEAD-box RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
259-264Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM133743
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Genes Dev. 1997 Jul 15;11(14):1864-72
pubmed: 9242493
Virology. 1995 Jan 10;206(1):116-25
pubmed: 7831767
Nature. 2006 Dec 21;444(7122):1078-82
pubmed: 17151603
Nat Microbiol. 2019 Oct;4(10):1671-1679
pubmed: 31263181
Sci Rep. 2017 Oct 25;7(1):14053
pubmed: 29070793
Nat Rev Microbiol. 2018 Jan 15;16(2):61-62
pubmed: 29332942
Nat Rev Microbiol. 2016 Aug;14(8):479-93
pubmed: 27396566
Cell. 2012 Nov 9;151(4):871-884
pubmed: 23141543
Traffic. 2019 Nov;20(11):829-840
pubmed: 31513326
J Virol. 2001 Feb;75(4):1899-908
pubmed: 11160689
EMBO J. 1994 Jul 1;13(13):3158-65
pubmed: 8039508
Evol Med Public Health. 2018 Sep 07;2018(1):192-198
pubmed: 30210800
Mol Cell. 2001 Sep;8(3):645-56
pubmed: 11583626
Viruses. 2016 Jun 17;8(6):
pubmed: 27322310
Annu Rev Biochem. 2015;84:325-54
pubmed: 25784054
RNA Biol. 2013 Aug;10(8):1274-82
pubmed: 23807439
J Gen Virol. 2010 Feb;91(Pt 2):313-28
pubmed: 19955561
J Virol. 2011 Jul;85(13):6197-204
pubmed: 21507964
J Virol. 1998 Jul;72(7):5493-501
pubmed: 9621005
J Am Soc Mass Spectrom. 2012 Jan;23(1):76-87
pubmed: 22038510
Elife. 2017 Jan 06;6:
pubmed: 28059701
Nat Rev Microbiol. 2015 Jan;13(1):28-41
pubmed: 25417656
Mol Cell. 1998 Apr;1(5):649-59
pubmed: 9660949
Nature. 2001 Oct 11;413(6856):644-7
pubmed: 11675789
Mol Cell Biol. 2008 Jan;28(2):601-8
pubmed: 17984224
Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1853-8
pubmed: 17267598
FASEB J. 2008 Oct;22(10):3638-47
pubmed: 18614582
Viruses. 2013 Jul 18;5(7):1824-49
pubmed: 23872491
Trends Biochem Sci. 2010 Nov;35(11):609-17
pubmed: 20719516
Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17628-33
pubmed: 15585580
Sci China C Life Sci. 2009 May;52(5):439-49
pubmed: 19471866
Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):505-16
pubmed: 21779027
Nat Commun. 2013;4:1591
pubmed: 23481399
J Mol Biol. 2003 Feb 21;326(3):849-58
pubmed: 12581645
Science. 2012 Dec 21;338(6114):1634-7
pubmed: 23180776
J Cell Sci. 2009 Jun 15;122(Pt 12):1933-7
pubmed: 19494120