Analysis of historical negative control group data from the rat in vivo micronucleus assay.
Historical negative control data
In vivo micronucleus assay
Quality control statistics
Wistar and Sprague Dawley rats
Journal
Mutation research. Genetic toxicology and environmental mutagenesis
ISSN: 1879-3592
Titre abrégé: Mutat Res Genet Toxicol Environ Mutagen
Pays: Netherlands
ID NLM: 101632149
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
08
07
2019
revised:
22
08
2019
accepted:
24
08
2019
entrez:
24
2
2020
pubmed:
24
2
2020
medline:
23
4
2020
Statut:
ppublish
Résumé
A database of micronuclei counts for historical negative control data from rat in vivo micronuclei tests performed in 10 different laboratories was established. Data were available from over 4000 negative control rats from 10 laboratories. The mean frequency of micronucleated cells (MN)/1000 cells ranged from 0.44 to 2.22, a 5-fold range. Overall there were no major sex or strain differences in frequency, although there were some small but statistically significant differences within laboratories. There was appreciable variability between experiments compared with variability within experiments in some laboratories. No specific factor was identified which could explain this variability although it was noted that many different vehicles were used in the experiments. It is hoped that these data will help laboratories beginning studies with the rat micronucleus assay and those involved in the assessment of micronucleus assay results.
Identifiants
pubmed: 32087845
pii: S1383-5718(19)30227-X
doi: 10.1016/j.mrgentox.2019.503086
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
503086Informations de copyright
Copyright © 2019. Published by Elsevier B.V.