MiR-34a Interacts with Cytochrome c and Shapes Stroke Outcomes.

Animals Blood-Brain Barrier / pathology Brain Ischemia / genetics Cytochromes c / metabolism Disease Models, Animal Mice, Inbred C57BL MicroRNAs / genetics Stroke / genetics Tight Junctions / metabolism Treatment Outcome

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 02 2020
Historique:
received: 22 11 2019
accepted: 04 02 2020
entrez: 26 2 2020
pubmed: 26 2 2020
medline: 13 11 2020
Statut: epublish

Résumé

Blood-brain barrier (BBB) dysfunction occurs in cerebrovascular diseases and neurodegenerative disorders such as stroke. Opening of the BBB during a stroke has a negative impact on acute outcomes. We have recently demonstrated that miR-34a regulates the BBB by targeting cytochrome c (CYC) in vitro. To investigate the role of miR-34a in a stroke, we purified primary cerebrovascular endothelial cells (pCECs) from mouse brains following 1 h transient middle cerebral artery occlusion (tMCAO) and measured real-time PCR to detect miR-34a levels. We demonstrate that the miR-34a levels are elevated in pCECs from tMCAO mice at the time point of BBB opening following 1 h tMCAO and reperfusion. Interestingly, knockout of miR-34a significantly reduces BBB permeability, alleviates disruption of tight junctions, and improves stroke outcomes compared to wild-type (WT) controls. CYC is decreased in the ischemic hemispheres and pCECs from WT but not in miR-34a

Identifiants

DOI: 10.1038/s41598-020-59997-y PMID: 32094435 PMC: PMC7040038
pubmed: 32094435
doi: 10.1038/s41598-020-59997-y
pii: 10.1038/s41598-020-59997-y
pmc: PMC7040038
doi:

Substances chimiques

MIRN34a microRNA, mouse 0
MicroRNAs 0
Cytochromes c 9007-43-6

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3233

Subventions

Organisme : NIGMS NIH HHS
ID : P20 GM109098
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL128485
Pays : United States
Organisme : NCRR NIH HHS
ID : P30 RR032138
Pays : United States
Organisme : NIGMS NIH HHS
ID : U54 GM104942
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM121322
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103434
Pays : United States
Organisme : NIGMS NIH HHS
ID : P30 GM103488
Pays : United States

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Auteurs

Heng Hu (H)

Departments of Physiology and Pharmacology, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.
Experimental Stroke Core, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

Emily A Hone (EA)

Neuroscience, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.
Microbiology, Immunology and Cell Biology, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

Edward A P Provencher (EAP)

Neuroscience, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

Samuel A Sprowls (SA)

Department of Basic Pharmaceutic Sciences, School of Pharmacy, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

Imran Farooqi (I)

Neuroscience, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

Deborah R Corbin (DR)

Neuroscience, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

Saumyendra N Sarkar (SN)

Departments of Physiology and Pharmacology, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

John M Hollander (JM)

Human Performance, School of Medicine, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

Paul R Lockman (PR)

Department of Basic Pharmaceutic Sciences, School of Pharmacy, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

James W Simpkins (JW)

Departments of Physiology and Pharmacology, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.
Experimental Stroke Core, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA.

Xuefang Ren (X)

Neuroscience, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA. xuren@hsc.wvu.edu.
Microbiology, Immunology and Cell Biology, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA. xuren@hsc.wvu.edu.
Human Performance, School of Medicine, Center for Basic and Translational Stroke Research; West Virginia University, Morgantown, West Virginia, 26506, USA. xuren@hsc.wvu.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Barrière hémato-encéphalique › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Maladies cardiovasculaires › Maladies vasculaires › Angiopathies intracrâniennes › Encéphalopathie ischémique › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Hémoprotéines › Cytochromes › Cytochromes de type c › Cytochromes c › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Maladies cardiovasculaires › Maladies vasculaires › Angiopathies intracrâniennes › Accident vasculaire cérébral › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Jonctions intercellulaires › Jonctions serrées › MiR-34a Interacts with Cytochrome c and Shapes...
questionsmedicales.fr › Qualité, accès, évaluation des soins de santé › Qualité des soins de santé › Mécanismes d'évaluation des soins de santé › Évaluation des résultats et des processus en soins de santé › Évaluation de résultat (soins) › Résultat thérapeutique › MiR-34a Interacts with Cytochrome c and Shapes...