Novel Expression of GABAA Receptors on Resistance Arteries That Modulate Myogenic Tone.
Animals
Arteries
/ drug effects
Calcium Signaling
Cells, Cultured
Female
GABA-A Receptor Agonists
/ pharmacology
GABA-A Receptor Antagonists
/ pharmacology
Male
Membrane Potentials
Mice, Inbred C57BL
Muscle, Smooth, Vascular
/ drug effects
Myocytes, Smooth Muscle
/ drug effects
Omentum
/ blood supply
Receptors, GABA-A
/ drug effects
Tail
/ blood supply
Vascular Resistance
Vasoconstriction
Vasodilation
GABA
Human omental artery
Membrane potential
Myogenic tone
Pressure myography
Resistance vessel
Journal
Journal of vascular research
ISSN: 1423-0135
Titre abrégé: J Vasc Res
Pays: Switzerland
ID NLM: 9206092
Informations de publication
Date de publication:
2020
2020
Historique:
received:
23
08
2019
accepted:
16
12
2019
pubmed:
26
2
2020
medline:
15
9
2020
entrez:
26
2
2020
Statut:
ppublish
Résumé
The clinical administration of GABAergic medications leads to hypotension which has classically been attributed to the modulation of neuronal activity in the central and peripheral nervous systems. However, certain types of peripheral smooth muscle cells have been shown to express GABAA receptors, which modulate smooth muscle tone, by the activation of these chloride channels on smooth muscle cell plasma membranes. Limited prior studies demonstrate that non-human large-caliber capacitance blood vessels mounted on a wire myograph are responsive to GABAA ligands. We questioned whether GABAA receptors are expressed in human resistance arteries and whether they modulate myogenic tone. We demonstrate the novel expression of GABAA subunits on vascular smooth muscle from small-caliber human omental and mouse tail resistance arteries. We show that GABAA receptors modulate both plasma membrane potential and calcium responses in primary cultured cells from human resistance arteries. Lastly, we demonstrate functional physiologic modulation of myogenic tone via GABAA receptor activation in human and mouse arteries. Together, these studies demonstrate a previously unrecognized role for GABAA receptors in the modulation of myogenic tone in mouse and human resistance arteries.
Identifiants
pubmed: 32097943
pii: 000505456
doi: 10.1159/000505456
pmc: PMC7228859
mid: NIHMS1069067
doi:
Substances chimiques
GABA-A Receptor Agonists
0
GABA-A Receptor Antagonists
0
Receptors, GABA-A
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113-125Subventions
Organisme : NIGMS NIH HHS
ID : L30 GM129791
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM065281
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008464
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD082251
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL124213
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL122340
Pays : United States
Informations de copyright
© 2020 S. Karger AG, Basel.
Références
Synapse. 1995 Nov;21(3):189-274
pubmed: 8578436
J Hypertens. 2015 Dec;33(12):2431-42
pubmed: 26536090
Acta Anaesthesiol Scand. 2000 Sep;44(8):1011-6
pubmed: 10981582
Am J Physiol Lung Cell Mol Physiol. 2008 Jun;294(6):L1206-16
pubmed: 18408071
Hypertension. 1993 Dec;22(6):913-21
pubmed: 8244524
Anesth Analg. 2004 Apr;98(4):1017-23, table of contents
pubmed: 15041591
Am J Physiol Lung Cell Mol Physiol. 2013 Feb 1;304(3):L191-7
pubmed: 23204068
PLoS One. 2015 Apr 01;10(4):e0122189
pubmed: 25830814
NCHS Data Brief. 2013 Oct;(133):1-8
pubmed: 24171916
J Cardiovasc Pharmacol. 1989 Sep;14(3):405-11
pubmed: 2476619
Am J Physiol Heart Circ Physiol. 2007 Jul;293(1):H628-35
pubmed: 17400711
J Vasc Res. 2010;47(3):183-202
pubmed: 19893316
Am J Respir Cell Mol Biol. 2016 Apr;54(4):546-53
pubmed: 26405827
J Biol Chem. 2006 Oct 6;281(40):29431-5
pubmed: 16901909
Int J Clin Exp Med. 2015 Aug 15;8(8):14296-302
pubmed: 26550413
Clin Sci (Lond). 2003 Dec;105(6):683-9
pubmed: 12877650
Anesthesiology. 2009 Apr;110(4):748-58
pubmed: 19322939
Clin Exp Pharmacol Physiol. 2002 Nov;29(11):1015-7
pubmed: 12366394
J Hypertens. 2005 Feb;23(2):247-50
pubmed: 15662208
J Clin Pharmacol. 2011 May;51(5):719-30
pubmed: 20547772
Anesth Analg. 1993 Apr;76(4):822-9
pubmed: 8466025
JAMA. 2003 Jul 9;290(2):199-206
pubmed: 12851274
Am J Physiol Lung Cell Mol Physiol. 2015 May 1;308(9):L931-42
pubmed: 25659897
Adv Physiol Educ. 2011 Mar;35(1):5-15
pubmed: 21385995
J Appl Physiol (1985). 2009 Apr;106(4):1257-63
pubmed: 19213928
Am J Physiol. 1993 Dec;265(6 Pt 2):H1847-55
pubmed: 8285222
Front Cell Neurosci. 2012 Jan 20;6:1
pubmed: 22319471
Mol Pharm. 2016 Jun 6;13(6):2026-38
pubmed: 27120014
J Cardiothorac Vasc Anesth. 2011 Feb;25(1):72-7
pubmed: 20599400
Circulation. 2015 Jan 27;131(4):e29-322
pubmed: 25520374
Gen Physiol Biophys. 2013 Mar;32(1):101-6
pubmed: 23531839
Am J Obstet Gynecol. 1997 Mar;176(3):560-6
pubmed: 9077607
J Vasc Res. 1997 Mar-Apr;34(2):109-16
pubmed: 9167643
Jpn J Pharmacol. 2001 Apr;85(4):351-7
pubmed: 11388637
Am J Physiol Heart Circ Physiol. 2002 Dec;283(6):H2226-33
pubmed: 12388224
Pharmacol Toxicol. 2001 Oct;89(4):183-7
pubmed: 11881968
Am J Physiol Lung Cell Mol Physiol. 2008 Dec;295(6):L1040-7
pubmed: 18790991
Hypertension. 2000 Jan;35(1 Pt 2):173-8
pubmed: 10642294