Sensing Actin Dynamics through Adherens Junctions.
actin
adhesion
cadherin
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
25 02 2020
25 02 2020
Historique:
received:
04
10
2019
revised:
23
12
2019
accepted:
29
01
2020
entrez:
27
2
2020
pubmed:
27
2
2020
medline:
18
3
2021
Statut:
ppublish
Résumé
We study punctate adherens junctions (pAJs) to determine how short-lived cadherin clusters and relatively stable actin bundles interact despite differences in dynamics. We show that pAJ-linked bundles consist of two distinct regions-the bundle stalk (AJ-BS) and a tip (AJ-BT) positioned between cadherin clusters and the stalk. The tip differs from the stalk in a number of ways: it is devoid of the actin-bundling protein calponin, and exhibits a much faster F-actin turnover rate. While F-actin in the stalk displays centripetal movement, the F-actin in the tip is immobile. The F-actin turnover in both the tip and stalk is dependent on cadherin cluster stability, which in turn is regulated by F-actin. The close bidirectional coupling between the stability of cadherin and associated F-actin shows how pAJs, and perhaps other AJs, allow cells to sense and coordinate the dynamics of the actin cytoskeleton in neighboring cells-a mechanism we term "dynasensing."
Identifiants
pubmed: 32101754
pii: S2211-1247(20)30150-9
doi: 10.1016/j.celrep.2020.01.106
pmc: PMC7093076
mid: NIHMS1566857
pii:
doi:
Substances chimiques
Actins
0
Cadherins
0
Microfilament Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2820-2833.e3Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR044016
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR070166
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118584
Pays : United States
Organisme : NIAMS NIH HHS
ID : R56 AR044016
Pays : United States
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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