The Transcriptome of Pig Spermatozoa, and Its Role in Fertility.
bioinformatics
fertility
microarrays
pig
spermatozoa
transcriptomics
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
25 Feb 2020
25 Feb 2020
Historique:
received:
19
02
2020
revised:
19
02
2020
accepted:
24
02
2020
entrez:
29
2
2020
pubmed:
29
2
2020
medline:
26
11
2020
Statut:
epublish
Résumé
In the study presented here we identified transcriptomic markers for fertility in the cargo of pig ejaculated spermatozoa using porcine-specific micro-arrays (GeneChip® miRNA 4.0 and GeneChip® Porcine Gene 1.0 ST). We report (i) the relative abundance of the ssc-miR-1285, miR-16, miR-4332, miR-92a, miR-671-5p, miR-4334-5p, miR-425-5p, miR-191, miR-92b-5p and miR-15b miRNAs, and (ii) the presence of 347 up-regulated and 174 down-regulated RNA transcripts in high-fertility breeding boars, based on differences of farrowing rate (FS) and litter size (LS), relative to low-fertility boars in the (Artificial Insemination) AI program. An overrepresentation analysis of the protein class (PANTHER) identified significant fold-increases for C-C chemokine binding (GO:0019957): CCR7, which activates B- and T-lymphocytes, 8-fold increase), XCR1 and CXCR4 (with ubiquitin as a natural ligand, 1.24-fold increase), cytokine receptor activity (GO:0005126): IL23R receptor of the IL23 protein, associated to JAK2 and STAT3, 3.4-fold increase), the TGF-receptor (PC00035) genes ACVR1C and ACVR2B (12-fold increase). Moreover, two micro-RNAs (miR-221 and mir-621) were down- and up-regulated, respectively, in high-fertility males. In conclusion, boars with different fertility performance possess a wide variety of differentially expressed RNA present in spermatozoa that would be attractive targets as non-invasive molecular markers for predicting fertility.
Identifiants
pubmed: 32106598
pii: ijms21051572
doi: 10.3390/ijms21051572
pmc: PMC7084236
pii:
doi:
Substances chimiques
Cytokines
0
MicroRNAs
0
STAT3 Transcription Factor
0
Janus Kinases
EC 2.7.10.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Svenska Forskningsrådet Formas
ID : Project 2017-00946
Organisme : Svenska Forskningsrådet Formas
ID : Project 2019-00288
Organisme : Ministerio de Economía y Competitividad
ID : AGL2015-69738-R
Organisme : Vetenskapsrådet
ID : Project 2015-05919
Organisme : Fundación Séneca
ID : 20780/PD/18
Déclaration de conflit d'intérêts
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
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