A Step-by-Step Protocol for Optogenetic Kindling.
Channelrhodopsin
animal model
epilepsy
kindling
optogenetics
protocol
seizure
Journal
Frontiers in neural circuits
ISSN: 1662-5110
Titre abrégé: Front Neural Circuits
Pays: Switzerland
ID NLM: 101477940
Informations de publication
Date de publication:
2020
2020
Historique:
received:
25
10
2019
accepted:
10
01
2020
entrez:
3
3
2020
pubmed:
3
3
2020
medline:
11
6
2021
Statut:
epublish
Résumé
Electrical kindling, repeated brain stimulation eventually resulting in seizures, is widely used as an animal model of epileptogenesis and epilepsy. However, the stimulation electrode used for electric kindling targets unknown neuronal populations and may introduce tissue damage and inflammation. Optogenetics can be used to circumvent these shortcomings by permitting millisecond control of activity in genetically defined neurons without gross injury or inflammation. Here we describe an easy step-by-step protocol for optogenetic kindling - optokindling - by which seizures are eventually elicited in initially healthy mice through repeated light stimulation of neurons expressing Channelrhodopsin-2 (ChR2). Chronic EEG recordings may be performed over large time scales to monitor activity while video camera monitoring may be used to assess the behavioral severity of seizures. In conclusion, with optokindling, neuroscientists can elucidate the circuit changes that underpin epilepsy while minimizing the contribution of confounding factors such as brain damage and inflammation.
Identifiants
pubmed: 32116570
doi: 10.3389/fncir.2020.00003
pmc: PMC7025532
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Technical Report
Langues
eng
Sous-ensembles de citation
IM
Pagination
3Subventions
Organisme : CIHR
ID : OG 126137
Pays : Canada
Organisme : CIHR
ID : PG 389378
Pays : Canada
Organisme : CIHR
ID : NIA 288936
Pays : Canada
Informations de copyright
Copyright © 2020 Cela and Sjöström.
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