Inactivation of Plasmodium falciparum in whole blood using the amustaline and glutathione pathogen reduction technology.
Journal
Transfusion
ISSN: 1537-2995
Titre abrégé: Transfusion
Pays: United States
ID NLM: 0417360
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
12
11
2019
revised:
16
01
2020
accepted:
04
02
2020
pubmed:
5
3
2020
medline:
9
9
2020
entrez:
5
3
2020
Statut:
ppublish
Résumé
Risk of transfusion-transmitted (TT) malaria is mainly associated with whole blood (WB) or red blood cell (RBC) transfusion. Risk mitigation relies mostly on donor deferral while a limited number of countries perform blood testing, both negatively impacting blood availability. This study investigated the efficacy of the pathogen reduction system using amustaline and glutathione (GSH) to inactivate Plasmodium falciparum in WB. WB units were spiked with ring stage P. falciparum infected RBCs. Parasite loads were measured in samples at time of infection, after 24 hours at room temperature (RT), and after a 24-hour incubation at RT post-treatment with 0.2 mM amustaline and 2 mM GSH. Serial 10-fold dilutions of the samples were inoculated to RBC cultures and maintained up to 4 weeks. Parasitemia was quantified by cytometry. The P. falciparum viability assay has a limit of detection of a single live parasite per sample. Input parasite titer was >5.7 log A robust level of P. falciparum inactivation was achieved in WB using amustaline/GSH treatment. Parasite log reduction was >5.7 log
Sections du résumé
BACKGROUND
Risk of transfusion-transmitted (TT) malaria is mainly associated with whole blood (WB) or red blood cell (RBC) transfusion. Risk mitigation relies mostly on donor deferral while a limited number of countries perform blood testing, both negatively impacting blood availability. This study investigated the efficacy of the pathogen reduction system using amustaline and glutathione (GSH) to inactivate Plasmodium falciparum in WB.
STUDY DESIGN AND METHODS
WB units were spiked with ring stage P. falciparum infected RBCs. Parasite loads were measured in samples at time of infection, after 24 hours at room temperature (RT), and after a 24-hour incubation at RT post-treatment with 0.2 mM amustaline and 2 mM GSH. Serial 10-fold dilutions of the samples were inoculated to RBC cultures and maintained up to 4 weeks. Parasitemia was quantified by cytometry.
RESULTS
The P. falciparum viability assay has a limit of detection of a single live parasite per sample. Input parasite titer was >5.7 log
CONCLUSION
A robust level of P. falciparum inactivation was achieved in WB using amustaline/GSH treatment. Parasite log reduction was >5.7 log
Identifiants
pubmed: 32129497
doi: 10.1111/trf.15734
pmc: PMC7187285
doi:
Substances chimiques
(N,N-bis(2-chloroethyl))-2-aminoethyl-3-((acridin-9-yl)amino)propionate
0
Acridines
0
Nitrogen Mustard Compounds
0
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
799-805Subventions
Organisme : Cerus Corporation
Pays : International
Organisme : Humanitarian Foundation Swiss Red Cross
Pays : International
Informations de copyright
© 2020 The Authors. Transfusion published by Wiley Periodicals, Inc. on behalf of AABB.
Références
Vox Sang. 2006 Feb;90(2):77-84
pubmed: 16430664
Emerg Infect Dis. 2002 Aug;8(8):872; author reply 873-4
pubmed: 12141979
Malar J. 2018 Jan 16;17(1):36
pubmed: 29338786
Nat Protoc. 2009;4(12):1899-915
pubmed: 20010926
Open Forum Infect Dis. 2019 Jun 11;6(7):ofz283
pubmed: 31334300
Hematol Oncol Clin North Am. 2016 Apr;30(2):477-95
pubmed: 27040966
Transfusion. 2008 Aug;48(8):1676-84
pubmed: 18503613
Transfusion. 2017 Oct;57(10):2404-2412
pubmed: 28872685
CMAJ. 2009 May 26;180(11):1129-31
pubmed: 19468122
Blood Transfus. 2019 Mar;17(2):94-102
pubmed: 30865584
PLoS Pathog. 2010 Feb 19;6(2):e1000770
pubmed: 20174609
J Exp Med. 1989 Jan 1;169(1):357-61
pubmed: 2642531
Transfus Med Hemother. 2011;38(1):33-42
pubmed: 21779204
Malar J. 2017 Feb 15;16(1):78
pubmed: 28202065
Biologicals. 2010 Jan;38(1):14-9
pubmed: 19995680
Methods Mol Biol. 2013;923:523-34
pubmed: 22990802
Transfusion. 2018 Mar;58(3):748-757
pubmed: 29322519
Blood Transfus. 2016 Mar;14(2):101-8
pubmed: 26509824
J Parasitol. 1979 Jun;65(3):418-20
pubmed: 383936
Transfusion. 2017 Dec;57(12):2888-2896
pubmed: 28921542
Malar J. 2013 Dec 28;12:465
pubmed: 24373501
Transfusion. 2013 Dec;53(12):3174-83
pubmed: 23656538
Lancet. 2016 Apr 23;387(10029):1753-61
pubmed: 27116282