Abundant HIV-infected cells in blood and tissues are rapidly cleared upon ART initiation during acute HIV infection.


Journal

Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086

Informations de publication

Date de publication:
04 03 2020
Historique:
received: 14 09 2018
revised: 19 09 2019
accepted: 21 01 2020
entrez: 6 3 2020
pubmed: 7 3 2020
medline: 24 6 2021
Statut: ppublish

Résumé

The timing and location of the establishment of the viral reservoir during acute HIV infection remain unclear. Using longitudinal blood and tissue samples obtained from HIV-infected individuals at the earliest stage of infection, we demonstrate that frequencies of infected cells reach maximal values in gut-associated lymphoid tissue and lymph nodes as early as Fiebig stage II, before seroconversion. Both tissues displayed higher frequencies of infected cells than blood until Fiebig stage III, after which infected cells were equally distributed in all compartments examined. Initiation of antiretroviral therapy (ART) at Fiebig stages I to III led to a profound decrease in the frequency of infected cells to nearly undetectable level in all compartments. The rare infected cells that persisted were preferentially found in the lymphoid tissues. Initiation of ART at later stages (Fiebig stages IV/V and chronic infection) induced only a modest reduction in the frequency of infected cells. Quantification of HIV DNA in memory CD4

Identifiants

pubmed: 32132218
pii: 12/533/eaav3491
doi: 10.1126/scitranslmed.aav3491
pmc: PMC7293182
mid: NIHMS1589647
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI108433
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI125127
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS061696
Pays : United States

Informations de copyright

Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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Auteurs

Louise Leyre (L)

Centre de Recherche du CHUM and Department of Microbiology, Infectiology and Immunology, Université de Montréal, Montréal, QC H2X 0A9, Canada.

Eugène Kroon (E)

SEARCH, Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand.

Claire Vandergeeten (C)

Vaccine and Gene Therapy Institute of Florida, FL 34987, USA.

Carlo Sacdalan (C)

SEARCH, Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand.

Donn J Colby (DJ)

SEARCH, Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand.

Supranee Buranapraditkun (S)

Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Alexandra Schuetz (A)

Department of Retrovirology, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.
U.S. Military HIV Research Program, Walter Reed Army Institute of Research Silver Spring, MD 20910, USA.

Nitiya Chomchey (N)

SEARCH, Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand.

Mark de Souza (M)

SEARCH, Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand.

Wendy Bakeman (W)

Vaccine and Gene Therapy Institute of Florida, FL 34987, USA.

Rémi Fromentin (R)

Centre de Recherche du CHUM and Department of Microbiology, Infectiology and Immunology, Université de Montréal, Montréal, QC H2X 0A9, Canada.

Suteeraporn Pinyakorn (S)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.
U.S. Military HIV Research Program, Walter Reed Army Institute of Research Silver Spring, MD 20910, USA.

Siriwat Akapirat (S)

Department of Retrovirology, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.

Rapee Trichavaroj (R)

Department of Retrovirology, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.

Suthat Chottanapund (S)

SEARCH, Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand.

Sopark Manasnayakorn (S)

Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Rungsun Rerknimitr (R)

Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Phandee Wattanaboonyoungcharoen (P)

Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Jerome H Kim (JH)

International Vaccine Institute, Seoul 08826, Korea.

Sodsai Tovanabutra (S)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.
U.S. Military HIV Research Program, Walter Reed Army Institute of Research Silver Spring, MD 20910, USA.

Timothy W Schacker (TW)

Department of Medicine, Medical School, University of Minnesota, Minneapolis, MN 55455, USA.

Robert O'Connell (R)

Department of Retrovirology, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.
U.S. Military HIV Research Program, Walter Reed Army Institute of Research Silver Spring, MD 20910, USA.

Victor G Valcour (VG)

University of California San Francisco, San Francisco, CA 94117, USA.

Praphan Phanuphak (P)

SEARCH, Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand.
Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Merlin L Robb (ML)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.
U.S. Military HIV Research Program, Walter Reed Army Institute of Research Silver Spring, MD 20910, USA.

Nelson Michael (N)

U.S. Military HIV Research Program, Walter Reed Army Institute of Research Silver Spring, MD 20910, USA.

Lydie Trautmann (L)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.
U.S. Military HIV Research Program, Walter Reed Army Institute of Research Silver Spring, MD 20910, USA.

Nittaya Phanuphak (N)

SEARCH, Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand.

Jintanat Ananworanich (J)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.
U.S. Military HIV Research Program, Walter Reed Army Institute of Research Silver Spring, MD 20910, USA.
Department of Global Health, University of Amsterdam, Amsterdam, Netherlands.

Nicolas Chomont (N)

Centre de Recherche du CHUM and Department of Microbiology, Infectiology and Immunology, Université de Montréal, Montréal, QC H2X 0A9, Canada. nicolas.chomont@umontreal.ca.

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