Disordered protein-graphene oxide co-assembly and supramolecular biofabrication of functional fluidic devices.

Animals Bioprinting / methods Cell Culture Techniques / methods Cell Line Chick Embryo Chorioallantoic Membrane Equipment Design / methods Graphite / chemistry Human Umbilical Vein Endothelial Cells Humans Hydrophobic and Hydrophilic Interactions Lab-On-A-Chip Devices Molecular Dynamics Simulation Printing, Three-Dimensional Protein Multimerization Protein Structure, Quaternary ets-Domain Protein Elk-1 / chemistry

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
04 03 2020
Historique:
received: 13 11 2019
accepted: 24 01 2020
entrez: 6 3 2020
pubmed: 7 3 2020
medline: 27 6 2020
Statut: epublish

Résumé

Supramolecular chemistry offers an exciting opportunity to assemble materials with molecular precision. However, there remains an unmet need to turn molecular self-assembly into functional materials and devices. Harnessing the inherent properties of both disordered proteins and graphene oxide (GO), we report a disordered protein-GO co-assembling system that through a diffusion-reaction process and disorder-to-order transitions generates hierarchically organized materials that exhibit high stability and access to non-equilibrium on demand. We use experimental approaches and molecular dynamics simulations to describe the underlying molecular mechanism of formation and establish key rules for its design and regulation. Through rapid prototyping techniques, we demonstrate the system's capacity to be controlled with spatio-temporal precision into well-defined capillary-like fluidic microstructures with a high level of biocompatibility and, importantly, the capacity to withstand flow. Our study presents an innovative approach to transform rational supramolecular design into functional engineering with potential widespread use in microfluidic systems and organ-on-a-chip platforms.

Identifiants

DOI: 10.1038/s41467-020-14716-z PMID: 32132534 PMC: PMC7055247
pubmed: 32132534
doi: 10.1038/s41467-020-14716-z
pii: 10.1038/s41467-020-14716-z
pmc: PMC7055247
doi:

Substances chimiques

ets-Domain Protein Elk-1 0
graphene oxide 0
Graphite 7782-42-5

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Video-Audio Media

Langues

eng

Sous-ensembles de citation

IM

Pagination

1182

Subventions

Organisme : Medical Research Council
ID : MR/R015651/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 108466/Z/15/Z
Pays : United Kingdom

Commentaires et corrections

Type : CommentIn

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Auteurs

Yuanhao Wu (Y)

Institute of Bioengineering, Queen Mary University of London, London, E1 4NS, UK.
School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.
School of Pharmacy, University of Nottingham, NG7 2RD, Nottingham, UK.
Department of Chemical and Environmental Engineering, University of Nottingham, NG7 2RD, Nottingham, UK.
Biodiscovery Institute, University of Nottingham, NG7 2RD, Nottingham, UK.
ORCID: 0000-0002-3955-6474

Babatunde O Okesola (BO)

Institute of Bioengineering, Queen Mary University of London, London, E1 4NS, UK.
School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.
ORCID: 0000-0003-0392-9205

Jing Xu (J)

Institute of Bioengineering, Queen Mary University of London, London, E1 4NS, UK.
School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.

Ivan Korotkin (I)

School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.
Mathematical Sciences, University of Southampton, Southampton SO17 1BJ, UK.

Alice Berardo (A)

Laboratory of Bio-inspired, Bionic, Nano, Meta Materials & Mechanics, Università di Trento, via Mesiano, 77, I-38123, Trento, Italy.
C3A - Center Agriculture Food Environment, University of Trento/Fondazione Edmund Mach, Via Edmund Mach, 1 - 38010, San Michele all'Adige (TN), Italy.
ORCID: 0000-0002-2346-0507

Ilaria Corridori (I)

Laboratory of Bio-inspired, Bionic, Nano, Meta Materials & Mechanics, Università di Trento, via Mesiano, 77, I-38123, Trento, Italy.
ORCID: 0000-0002-7348-9578

Francesco Luigi Pellerej di Brocchetti (FLP)

Research Center'E. Piaggio' & Dipartimento di Ingegneria dell'Informazione, University of Pisa, Largo Lucio Lazzarino, 256126, Pisa, Italy.

Janos Kanczler (J)

Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK.

Jingyu Feng (J)

School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.

Weiqi Li (W)

Institute of Bioengineering, Queen Mary University of London, London, E1 4NS, UK.
School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.

Yejiao Shi (Y)

Institute of Bioengineering, Queen Mary University of London, London, E1 4NS, UK.
School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.
ORCID: 0000-0002-1648-0833

Vladimir Farafonov (V)

Department of Physical Chemistry, V. N. Karazin Kharkiv National University, Svobody Sq. 4, Kharkiv, 61022, Ukraine.
ORCID: 0000-0003-0785-9582

Yiqiang Wang (Y)

United Kingdom Atomic Energy Authority, Culham Science Centre, Abingdon, OX14 3DB, UK.

Rebecca F Thompson (RF)

The Astbury Biostructure Laboratory, Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK.
ORCID: 0000-0002-4254-8854

Maria-Magdalena Titirici (MM)

School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.
ORCID: 0000-0003-0773-2100

Dmitry Nerukh (D)

Systems Analytics Research Institute, Department of Mathematics, Aston University, Birmingham, B4 7ET, UK.
ORCID: 0000-0001-9005-9919

Sergey Karabasov (S)

School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.

Richard O C Oreffo (ROC)

Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, SO16 6YD, UK.
ORCID: 0000-0001-5995-6726

Jose Carlos Rodriguez-Cabello (J)

BIOFORGE Group, University of Valladolid, CIBER-BBN, 47011, Valladolid, Spain.

Giovanni Vozzi (G)

Research Center'E. Piaggio' & Dipartimento di Ingegneria dell'Informazione, University of Pisa, Largo Lucio Lazzarino, 256126, Pisa, Italy.
ORCID: 0000-0002-9414-9994

Helena S Azevedo (HS)

Institute of Bioengineering, Queen Mary University of London, London, E1 4NS, UK.
School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.
ORCID: 0000-0002-5470-1844

Nicola M Pugno (NM)

School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.
Laboratory of Bio-inspired, Bionic, Nano, Meta Materials & Mechanics, Università di Trento, via Mesiano, 77, I-38123, Trento, Italy.
KET Labs, Edoardo Amaldi Foundation, Via del Politecnico snc, 00133, Rome, Italy.
ORCID: 0000-0003-2136-2396

Wen Wang (W)

Institute of Bioengineering, Queen Mary University of London, London, E1 4NS, UK.
School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.

Alvaro Mata (A)

Institute of Bioengineering, Queen Mary University of London, London, E1 4NS, UK. a.mata@nottingham.ac.uk.
School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK. a.mata@nottingham.ac.uk.
School of Pharmacy, University of Nottingham, NG7 2RD, Nottingham, UK. a.mata@nottingham.ac.uk.
Department of Chemical and Environmental Engineering, University of Nottingham, NG7 2RD, Nottingham, UK. a.mata@nottingham.ac.uk.
Biodiscovery Institute, University of Nottingham, NG7 2RD, Nottingham, UK. a.mata@nottingham.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Bio-impression › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Structures de l'embryon › Embryon non mammalien › Embryon de poulet › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Structures de l'embryon › Embryon non mammalien › Chorioallantoïde › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Techniques d'investigation › Conception d'appareillage › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Produits chimiques inorganiques › Minéraux › Graphite › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules endothéliales › Cellules endothéliales de la veine ombilicale humaine › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Phénomènes chimiques › Interactions hydrophobes et hydrophiles › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures électriques › Laboratoires sur puces › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de dynamique moléculaire › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Sciences de l'information › Affichage de données › Infographie › Conception assistée par ordinateur › Impression tridimensionnelle › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Multimérisation de protéines › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure quaternaire des protéines › Disordered protein-graphene oxide co-assembly...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines proto-oncogènes c-ets › Facteurs du complexe ternaire › Protéine Elk-1 à domaine ets › Disordered protein-graphene oxide co-assembly...