Improved third-generation lentiviral packaging with pLKO.1C vectors.
lentivirus
pLKO.1
shRNA
third-generation viral packaging
Journal
BioTechniques
ISSN: 1940-9818
Titre abrégé: Biotechniques
Pays: England
ID NLM: 8306785
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
pubmed:
7
3
2020
medline:
1
9
2021
entrez:
7
3
2020
Statut:
ppublish
Résumé
A widely used third-generation lentiviral packaging system produces virus with enhanced biosafety by eliminating HIV accessory genes and separating packaging elements into three different plasmids. However, for certain vectors such as pLKO.1, third-generation safety features reduce lentiviral titers due to the lack of the accessory gene
Identifiants
pubmed: 32141762
doi: 10.2144/btn-2019-0155
pmc: PMC7333533
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
349-352Subventions
Organisme : NCI NIH HHS
ID : R01 CA137124
Pays : United States
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