Detection of Active Granzyme A in NK92 Cells with Fluorescent Activity-Based Probe.
Journal
Journal of medicinal chemistry
ISSN: 1520-4804
Titre abrégé: J Med Chem
Pays: United States
ID NLM: 9716531
Informations de publication
Date de publication:
26 03 2020
26 03 2020
Historique:
pubmed:
7
3
2020
medline:
15
9
2020
entrez:
7
3
2020
Statut:
ppublish
Résumé
Cytotoxic T-lymphocytes (CTLs) and natural killer cells (NKs) kill compromised cells to defend against tumor and viral infections. Both effector cell types use multiple strategies to induce target cell death including Fas/CD95 activation and the release of perforin and a group of lymphocyte granule serine proteases called granzymes. Granzymes have relatively broad and overlapping substrate specificities and may hydrolyze a wide range of peptidic epitopes; it is therefore challenging to identify their natural and synthetic substrates and to distinguish their localization and functions. Here, we present a specific and potent substrate, an inhibitor, and an activity-based probe of Granzyme A (GrA) that can be used to follow functional GrA in cells.
Identifiants
pubmed: 32142286
doi: 10.1021/acs.jmedchem.9b02042
pmc: PMC7590976
doi:
Substances chimiques
Coumarins
0
Fluorescent Dyes
0
Oligopeptides
0
Serine Proteinase Inhibitors
0
Granzymes
EC 3.4.21.-
GZMA protein, human
EC 3.4.21.78
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3359-3369Références
Mol Cell Biol. 2002 Apr;22(8):2810-20
pubmed: 11909973
Nat Struct Biol. 2003 Jul;10(7):527-34
pubmed: 12819769
Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2518-23
pubmed: 24550277
Blood. 2009 Aug 6;114(6):1205-16
pubmed: 19506301
Nat Immunol. 2003 Feb;4(2):145-53
pubmed: 12524539
Cell. 2003 Mar 7;112(5):659-72
pubmed: 12628186
J Biol Chem. 2001 Feb 2;276(5):3683-90
pubmed: 11060286
Biochem Biophys Res Commun. 1999 Aug 2;261(2):251-5
pubmed: 10425174
Proc Natl Acad Sci U S A. 2001 May 8;98(10):5746-51
pubmed: 11331782
J Biol Chem. 2014 Mar 28;289(13):9408-17
pubmed: 24505135
Biol Chem. 2010 Aug;391(8):983-97
pubmed: 20536382
J Am Chem Soc. 2017 Jul 26;139(29):10115-10125
pubmed: 28672107
Mol Cell Biol. 1998 Nov;18(11):6387-98
pubmed: 9774654
Cell. 1987 Jun 5;49(5):679-85
pubmed: 3555842
Nat Protoc. 2017 Oct;12(10):2189-2214
pubmed: 28933778
Mol Cell. 2006 Jul 7;23(1):133-42
pubmed: 16818237
Chem Biol. 2005 May;12(5):567-77
pubmed: 15911377
J Med Chem. 2018 Jun 28;61(12):5222-5234
pubmed: 29806773
Mol Immunol. 1988 Dec;25(12):1283-9
pubmed: 3266293
J Immunol. 1988 Nov 15;141(10):3471-7
pubmed: 3263427
Immunity. 2008 Nov 14;29(5):720-33
pubmed: 18951048
Immunol Cell Biol. 2006 Feb;84(1):79-86
pubmed: 16405655
Nat Struct Biol. 2003 Jul;10(7):535-40
pubmed: 12819770
Trends Immunol. 2012 Aug;33(8):406-12
pubmed: 22608996
J Biol Chem. 1988 Sep 15;263(26):13215-22
pubmed: 3047119
J Biol Chem. 1996 Nov 1;271(44):27802-9
pubmed: 8910377
Biol Chem. 2009 May-Jun;390(5-6):401-7
pubmed: 19361286
Nature. 2010 Nov 18;468(7322):447-51
pubmed: 21037563
Biol Chem. 2006 Jul;387(7):827-37
pubmed: 16913832
PLoS One. 2015 Jul 14;10(7):e0132818
pubmed: 26172376
Methods Enzymol. 1994;244:423-41
pubmed: 7845224
Immunol Rev. 2010 May;235(1):93-104
pubmed: 20536557
Cell Death Differ. 2014 Sep;21(9):1482-92
pubmed: 24832467
Mol Immunol. 1996 May-Jun;33(7-8):615-23
pubmed: 8760273