Housekeeping Gene Expression in the Fetal and Neonatal Murine Thymus Following Coxsackievirus B4 Infection.
Coxsackievirus B4
housekeeping genes
in utero infection
swiss albino mice
thymic epithelial cells
thymus development
Journal
Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097
Informations de publication
Date de publication:
05 03 2020
05 03 2020
Historique:
received:
16
12
2019
revised:
18
01
2020
accepted:
05
02
2020
entrez:
11
3
2020
pubmed:
11
3
2020
medline:
20
1
2021
Statut:
epublish
Résumé
The thymus fulfills the role of T-cell production and differentiation. Studying transcription factors and genes involved in T-cell differentiation and maturation during the fetal and neonatal periods is very important. Nevertheless, no studies to date have been interested in evaluating the expressions of housekeeping genes as internal controls to assess the varying expressions of different genes inside this tissue during that period or in the context of viral infection. Thus, we evaluated by real-time quantitative polymerase chain reaction (qPCR) the expression of the most common internal control genes in the thymus of Swiss albino mice during the fetal and neonatal period, and following in utero infection with Coxsackievirus B4. The stability of expression of these reference genes in different samples was investigated using the geNorm application. Results demonstrated that the expression stability varied greatly between genes.
Identifiants
pubmed: 32150956
pii: genes11030279
doi: 10.3390/genes11030279
pmc: PMC7140870
pii:
doi:
Substances chimiques
Proteins
0
ornithine decarboxylase antizyme
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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