Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons.
Adaptor Proteins, Signal Transducing
/ antagonists & inhibitors
Amino Acid Motifs
Animals
Binding, Competitive
Cells, Cultured
Dendritic Spines
/ physiology
Disks Large Homolog 4 Protein
/ physiology
Gene Knockdown Techniques
Hippocampus
/ cytology
Microtubules
/ metabolism
Neuronal Plasticity
/ physiology
Protein Binding
Protein Interaction Mapping
RNA Interference
RNA, Small Interfering
/ genetics
Rats
Arborization
CRIPT
Dendrite
Dendritic spine
Morphology
Neuron
Journal
Molecular neurobiology
ISSN: 1559-1182
Titre abrégé: Mol Neurobiol
Pays: United States
ID NLM: 8900963
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
09
10
2019
accepted:
24
02
2020
pubmed:
12
3
2020
medline:
4
2
2021
entrez:
12
3
2020
Statut:
ppublish
Résumé
CRIPT, the cysteine-rich PDZ-binding protein, binds to the third PDZ domain of PSD-95 (postsynaptic density protein 95) family proteins and directly binds microtubules, linking PSD-95 family proteins to the neuronal cytoskeleton. Here, we show that overexpression of a full-length CRIPT leads to a modest decrease, and knockdown of CRIPT leads to an increase in dendritic branching in cultured rat hippocampal neurons. Overexpression of truncated CRIPT lacking the PDZ domain-binding motif, which does not bind to PSD-95, significantly decreases dendritic arborization. Conversely, overexpression of a full-length CRIPT significantly increases the number of immature and mature dendritic spines, and this effect is not observed when CRIPT∆PDZ is overexpressed. Competitive inhibition of CRIPT binding to the third PDZ domain of PSD-95 with PDZ3-binding peptides resulted in differential effects on dendritic arborization based on the origin of respective peptide sequence. These results highlight multifunctional roles of CRIPT during development and underscore the significance of the interaction between CRIPT and the third PDZ domain of PSD-95.
Identifiants
pubmed: 32157575
doi: 10.1007/s12035-020-01895-5
pii: 10.1007/s12035-020-01895-5
pmc: PMC7176523
mid: NIHMS1574490
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Cript protein, rat
0
Disks Large Homolog 4 Protein
0
Dlg4 protein, rat
0
RNA, Small Interfering
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2479-2493Subventions
Organisme : NIGMS NIH HHS
ID : K12 GM093854
Pays : United States
Organisme : NIH HHS
ID : Biotechnology Training Grant T32 GM008339-20
Pays : United States
Organisme : NIH HHS
ID : IRACDA (Institutional Research and Career Development Award) INSPIRE - IRACDA2K12GM093854-07A1
Pays : United States
Organisme : New Jersey Commission on Brain Injury Research
ID : CBIR14IRG019
Organisme : NIGMS NIH HHS
ID : T32 GM008339
Pays : United States
Organisme : New Jersey Commission on Brain Injury Research
ID : CBIR19FEL018
Organisme : National Science Foundation
ID : IOS-1353724
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