Enzymes with noncanonical amino acids.

Amino Acid Sequence Amino Acids / chemistry Amino Acyl-tRNA Synthetases / metabolism Biocatalysis Catalytic Domain Diazonium Compounds / chemistry Enzymes / chemistry Genetic Code Molecular Conformation Oxidation-Reduction Photochemical Processes Protein Engineering beta-Alanine / analogs & derivatives
Directed evolution Enzyme design Genetic code expansion Noncanonical amino acids

Journal

Current opinion in chemical biology
ISSN: 1879-0402
Titre abrégé: Curr Opin Chem Biol
Pays: England
ID NLM: 9811312

Informations de publication

Date de publication:
04 2020
Historique:
received: 10 10 2019
revised: 10 12 2019
accepted: 15 01 2020
pubmed: 13 3 2020
medline: 13 2 2021
entrez: 13 3 2020
Statut: ppublish

Résumé

Enzyme design and engineering strategies rely almost exclusively on nature's alphabet of twenty canonical amino acids. Recent years have seen the emergence of powerful genetic code expansion methods that allow hundreds of structurally diverse amino acids to be installed into proteins in a site-selective manner. Here, we will highlight how the availability of an expanded alphabet of amino acids has opened new avenues in enzyme engineering research. Genetically encoded noncanonical amino acids have provided new tools to probe complex enzyme mechanisms, improve biocatalyst activity and stability, and most ambitiously to design enzymes with new catalytic mechanisms that would be difficult to access within the constraints of the genetic code. We anticipate that the studies highlighted in this article, coupled with the continuing advancements in genetic code expansion technology, will promote the widespread use of noncanonical amino acids in biocatalysis research in the coming years.

Identifiants

DOI: 10.1016/j.cbpa.2020.01.006 PMID: 32163871
pubmed: 32163871
pii: S1367-5931(20)30007-7
doi: 10.1016/j.cbpa.2020.01.006
pii:
doi:

Substances chimiques

Amino Acids 0
Diazonium Compounds 0
Enzymes 0
beta-Alanine 11P2JDE17B
2,3-diaminopropionic acid 8908T5BN6R
Amino Acyl-tRNA Synthetases EC 6.1.1.-
diazoacetic ester N84B835FMR

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

136-144

Subventions

Organisme : European Research Council
ID : 757991
Pays : International
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M027023/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M027023/1
Pays : United Kingdom

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest statement Nothing declared.

Auteurs

Jingming Zhao (J)

Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.

Ashleigh J Burke (AJ)

Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.

Anthony P Green (AP)

Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. Electronic address: anthony.green@manchester.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Carbon-oxygen ligases › Amino acyl-tRNA synthetases › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Métabolisme › Biocatalyse › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Composés chimiques organiques › Composés azoïques › Composés diazonium › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Code génétique › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Phénomènes chimiques › Processus photochimiques › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Enzymes with noncanonical amino acids.
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Alanine › bêta-Alanine › Enzymes with noncanonical amino acids.