Intrinsic alterations in peripheral neutrophils from cystic fibrosis newborn piglets.
CFTR
Cystic fibrosis
ICM-MS
Neutrophils
Pig model
Journal
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
ISSN: 1873-5010
Titre abrégé: J Cyst Fibros
Pays: Netherlands
ID NLM: 101128966
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
16
12
2019
revised:
22
02
2020
accepted:
23
02
2020
pubmed:
14
3
2020
medline:
21
10
2021
entrez:
14
3
2020
Statut:
ppublish
Résumé
The hallmark of the cystic fibrosis (CF) lung disease is a neutrophil dominated lung environment that is associated to chronic lung tissue destruction and ultimately the patient's death. It is unclear whether the exacerbated neutrophil response is primary related to a defective CFTR or rather secondary to chronic bacterial colonization and inflammation. Here, we hypothesized that CF peripheral blood neutrophils present intrinsic alteration at birth before the start of an inflammatory process. Peripheral blood neutrophils were isolated from newborn CFTR Polyunsaturated fatty acid metabolites analysis did not show any difference between CFTR ICM-MS demonstrated that CFTR
Sections du résumé
BACKGROUND
The hallmark of the cystic fibrosis (CF) lung disease is a neutrophil dominated lung environment that is associated to chronic lung tissue destruction and ultimately the patient's death. It is unclear whether the exacerbated neutrophil response is primary related to a defective CFTR or rather secondary to chronic bacterial colonization and inflammation. Here, we hypothesized that CF peripheral blood neutrophils present intrinsic alteration at birth before the start of an inflammatory process.
METHODS
Peripheral blood neutrophils were isolated from newborn CFTR
RESULTS
Polyunsaturated fatty acid metabolites analysis did not show any difference between CFTR
CONCLUSIONS
ICM-MS demonstrated that CFTR
Identifiants
pubmed: 32165155
pii: S1569-1993(20)30065-5
doi: 10.1016/j.jcf.2020.02.016
pii:
doi:
Substances chimiques
Fatty Acids, Unsaturated
0
Reactive Oxygen Species
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
830-836Informations de copyright
Copyright © 2020 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no conflict of interest.