Effect of helical kink in antimicrobial peptides on membrane pore formation.
antibiotics
fluorescent probes
membrane structure
membrane transport
molecular biophysics
none
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
13 03 2020
13 03 2020
Historique:
received:
25
04
2019
accepted:
18
02
2020
entrez:
14
3
2020
pubmed:
14
3
2020
medline:
31
3
2021
Statut:
epublish
Résumé
Every cell is protected by a semipermeable membrane. Peptides with the right properties, for example Antimicrobial peptides (AMPs), can disrupt this protective barrier by formation of leaky pores. Unfortunately, matching peptide properties with their ability to selectively form pores in bacterial membranes remains elusive. In particular, the proline/glycine kink in helical peptides was reported to both increase and decrease antimicrobial activity. We used computer simulations and fluorescence experiments to show that a kink in helices affects the formation of membrane pores by stabilizing toroidal pores but disrupting barrel-stave pores. The position of the proline/glycine kink in the sequence further controls the specific structure of toroidal pore. Moreover, we demonstrate that two helical peptides can form a kink-like connection with similar behavior as one long helical peptide with a kink. The provided molecular-level insight can be utilized for design and modification of pore-forming antibacterial peptides or toxins.
Identifiants
pubmed: 32167466
doi: 10.7554/eLife.47946
pii: 47946
pmc: PMC7069690
doi:
pii:
Substances chimiques
Antimicrobial Cationic Peptides
0
Porins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Czech Science Foundation
ID : GA17-11571S
Organisme : Czech Science Foundation
ID : 19-26854X
Organisme : Ministry of Education, Youth and Sports of the Czech Republic
ID : LQ1601
Organisme : Ministry of Education, Youth and Sports of the Czech Republic
ID : LM2015070
Organisme : Ministry of Education, Youth and Sports of the Czech Republic
ID : LM2015085
Organisme : Ministry of Education, Youth and Sports of the Czech Republic
ID : LM2015042
Informations de copyright
© 2020, Tuerkova et al.
Déclaration de conflit d'intérêts
AT, IK, TK, LS, ŠP, MH, RV No competing interests declared
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