Optimization of fermentation conditions for the production of epothilone B.
Epothilone B
fermentation conditions
optimization
orthogonal experiment
Journal
Chemical biology & drug design
ISSN: 1747-0285
Titre abrégé: Chem Biol Drug Des
Pays: England
ID NLM: 101262549
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
25
11
2019
revised:
09
02
2020
accepted:
15
02
2020
pubmed:
14
3
2020
medline:
23
6
2021
entrez:
14
3
2020
Statut:
ppublish
Résumé
Epothilone is a macrolide secondary metabolite which has the same anticancer effect as paclitaxel. Based on a series of single-factor experiments, four factors, temperature, initial pH, rotation speed, and inoculum quantity, which have the greatest influence on yield, were determined. Four factors were designed and orthogonal experiments were carried out to optimize the fermentation conditions. Finally, the best experimental conditions were obtained as follows: 250 ml flapper triangular flask was used. The yield of epothilone B was 39.76 mg/L at 30℃, initial pH = 7.4, rotating speed 200 r/min, inoculation amount 10%, liquid loading amount 50 ml/250 ml, fermentation time 6 days and seed age 60 hr.
Substances chimiques
Antineoplastic Agents
0
Epothilones
0
Oxygen
S88TT14065
epothilone B
UEC0H0URSE
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
768-772Informations de copyright
© 2020 John Wiley & Sons A/S.
Références
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