Multiplexed Gene Expression Tuning with Orthogonal Synthetic Gene Circuits.
2-dimensional
gene expression control
low variability
mammalian synthetic gene circuits
orthogonal control
synthetic biology
tunable
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
17 04 2020
17 04 2020
Historique:
pubmed:
14
3
2020
medline:
12
1
2021
entrez:
14
3
2020
Statut:
ppublish
Résumé
Understanding the individual and joint contribution of multiple protein levels toward a phenotype requires precise and tunable multigene expression control. Here we introduce a pair of mammalian synthetic gene circuits that linearly and orthogonally control the expression of two reporter genes in mammalian cells with low variability in response to chemical inducers introduced into the growth medium. These gene expression systems can be used to simultaneously probe the individual and joint effects of two gene product concentrations on a cellular phenotype in basic research or biomedical applications.
Identifiants
pubmed: 32167761
doi: 10.1021/acssynbio.9b00534
pmc: PMC7197936
mid: NIHMS1590488
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
930-939Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM122561
Pays : United States
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