Fabrication of microfluidic device for Aflatoxin M1 detection in milk samples with specific aptamers.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 03 2020
13 03 2020
Historique:
received:
07
01
2020
accepted:
05
02
2020
entrez:
15
3
2020
pubmed:
15
3
2020
medline:
15
12
2020
Statut:
epublish
Résumé
This study describes the colorimetric detection of aflatoxin M1 (Afl M1) in milk samples using a microfluidic paper-based analytical device (µPAD). Fabrication of µPADs was done using a simple and quick approach. Each μPAD contained a detection zone and a sample zone interconnected by microchannels. The colorimetric assay was developed using unmodified AuNPs as a probe and 21-mer aptamer as a recognition molecule. The free aptamers were adsorbed onto the surface of AuNPs in absence of Afl M1, even at high salt concentrations. The salt induced aggregation of specific aptamers occurred in presence of Afl M1. Under optimum conditions, the analytical linear range was found to be 1 µM to 1 pM with limit of detection 3 pM and 10 nM in standard buffer and spiked milk samples respectively. The proposed aptamer based colorimetric assay was repeatable, quick, selective, and can be used for on-site detection of other toxins in milk and meat samples.
Identifiants
pubmed: 32170077
doi: 10.1038/s41598-020-60926-2
pii: 10.1038/s41598-020-60926-2
pmc: PMC7070014
doi:
Substances chimiques
Aptamers, Nucleotide
0
Aflatoxin M1
6795-23-9
Gold
7440-57-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4627Subventions
Organisme : NIEHS NIH HHS
ID : 27302C0038
Pays : United States
Commentaires et corrections
Type : ErratumIn
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pubmed: 28367449
pmcid: 5358459