Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells.
Active Transport, Cell Nucleus
/ genetics
Autoantigens
/ chemistry
Binding Sites
Crystallography, X-Ray
HeLa Cells
Humans
Neoplasms
/ metabolism
Nuclear Export Signals
Phylogeny
Protein Binding
/ genetics
Protein Conformation, alpha-Helical
Protein Domains
Protein Interaction Maps
/ genetics
Ribonucleoproteins
/ chemistry
Tankyrases
/ chemistry
Transfection
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
13 03 2020
13 03 2020
Historique:
received:
30
10
2019
accepted:
21
02
2020
entrez:
15
3
2020
pubmed:
15
3
2020
medline:
10
2
2021
Statut:
epublish
Résumé
Sjögren syndrome/scleroderma autoantigen 1 (SSSCA1) was first described as an auto-antigen over-expressed in Sjögren's syndrome and in scleroderma patients. SSSCA1 has been linked to mitosis and centromere association and as a potential marker candidate in diverse solid cancers. Here we characterize SSSCA1 for the first time, to our knowledge, at the molecular, structural and subcellular level. We have determined the crystal structure of a zinc finger fold, a zinc ribbon domain type 2 (ZNRD2), at 2.3 Å resolution. We show that the C-terminal domain serves a dual function as it both behaves as the interaction site to Tankyrase 1 (TNKS1) and as a nuclear export signal. We identify TNKS1 as a direct binding partner of SSSCA1, map the binding site to TNKS1 ankyrin repeat cluster 2 (ARC2) and thus define a new binding sequence. We experimentally verify and map a new nuclear export signal sequence in SSSCA1.
Identifiants
pubmed: 32170109
doi: 10.1038/s42003-020-0851-2
pii: 10.1038/s42003-020-0851-2
pmc: PMC7070046
doi:
Substances chimiques
Autoantigens
0
Nuclear Export Signals
0
Ribonucleoproteins
0
SS-A antigen
0
Tankyrases
EC 2.4.2.30
TNKS protein, human
EC 2.4.4.30
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
123Références
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