Foxc2 Alleviates Ox-LDL-Induced Lipid Accumulation, Inflammation, and Apoptosis of Macrophage via Regulating the Expression of Angptl2.
Angiopoietin-Like Protein 2
Angiopoietin-like Proteins
/ biosynthesis
Animals
Apoptosis
/ drug effects
Forkhead Transcription Factors
/ biosynthesis
Gene Expression
Inflammation
/ chemically induced
Lipid Metabolism
/ drug effects
Lipoproteins, LDL
/ toxicity
Macrophages
/ drug effects
Mice
RAW 264.7 Cells
Angptl2
Apoptosis
Foxc2
Inflammation
Lipid accumulation
Journal
Inflammation
ISSN: 1573-2576
Titre abrégé: Inflammation
Pays: United States
ID NLM: 7600105
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
pubmed:
15
3
2020
medline:
11
6
2021
entrez:
15
3
2020
Statut:
ppublish
Résumé
The present study aimed to investigate the role of Forkhead box protein C2 (Foxc2) in oxidized low-density lipoprotein (ox-LDL)-induced macrophages and identify the potential mechanisms. RAW264.7 cells, the murine macrophage cell line, were stimulated by ox-LDL, and cell proliferation was examined. The levels of inflammation- and oxidative stress-related markers were detected using kits after induction with ox-LDL. Subsequently, the expression of Foxc2 was measured using Western blotting. After transfection with Foxc2 pcDNA3.1, intracellular lipid droplets were examined using oil red O staining. The levels of total cholesterol (TC), free cholesterol (FC), inflammatory cytokines, and oxidative stress markers were determined. Moreover, apoptosis of RAW264.7 cells was detected using flow cytometry, and apoptosis-related proteins were measured using Western blotting. Angiopoietin-like protein 2 (Angptl2) was predicted as a target gene of Foxc2. Therefore, the expression of Angptl2 was examined after Foxc2 overexpression in ox-LDL-induced RAW264.7 cells. Then, the changes of intracellular lipid droplets, TC, FC, inflammatory cytokines, oxidative stress factors, and cell apoptosis were detected after Angptl2 overexpression or co-transfection with Foxc2 and Angptl2 pcDNA3.1. The results revealed that ox-LDL induction inhibited proliferation of RAW264.7 cells and promoted the release of inflammatory factors. Importantly, the expression of Foxc2 was obviously decreased after stimulation by ox-LDL. Foxc2 overexpression suppressed lipid accumulation, TC, FC levels, inflammation, oxidative stress, and apoptosis induced by ox-LDL, whereas these inhibitory effects were relieved after co-transfection with Angptl2 pcDNA3.1. These findings demonstrated that Foxc2 can alleviate ox-LDL-induced lipid accumulation, inflammation, and apoptosis of macrophage via regulating the expression of Angptl2.
Identifiants
pubmed: 32170602
doi: 10.1007/s10753-020-01217-w
pii: 10.1007/s10753-020-01217-w
doi:
Substances chimiques
Angiopoietin-Like Protein 2
0
Angiopoietin-like Proteins
0
Angptl2 protein, mouse
0
Forkhead Transcription Factors
0
Lipoproteins, LDL
0
mesenchyme fork head 1 protein
0
oxidized low density lipoprotein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1397-1410Subventions
Organisme : the Natural Science Foundation of Hunan Province, China
ID : 14JJ7006
Organisme : the Science and Technology Innovation Planning Project of Hunan Province
ID : 2017SK50104
Organisme : the Scientific Research Project of Hunan Health Commission
ID : 20201228
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