Age Distribution of Multiple Functionally Relevant Subsets of CD4+ T Cells in Human Blood Using a Standardized and Validated 14-Color EuroFlow Immune Monitoring Tube.
Adolescent
Adult
Age Distribution
Aged
Aged, 80 and over
Blood Donors
CD4-Positive T-Lymphocytes
/ immunology
Child
Child, Preschool
Female
Fetal Blood
/ cytology
Humans
Immunophenotyping
/ methods
Infant
Male
Middle Aged
Monitoring, Immunologic
/ methods
Phenotype
Reproducibility of Results
T-Lymphocytes, Helper-Inducer
/ immunology
T-Lymphocytes, Regulatory
/ immunology
Young Adult
CD4+ T-cell subsets
TFH
Th-cell subsets
Tregs
age-related values
flow cytometry
immune monitoring
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
02
09
2019
accepted:
21
01
2020
entrez:
17
3
2020
pubmed:
17
3
2020
medline:
9
3
2021
Statut:
epublish
Résumé
CD4+ T cells comprise multiple functionally distinct cell populations that play a key role in immunity. Despite blood monitoring of CD4+ T-cell subsets is of potential clinical utility, no standardized and validated approaches have been proposed so far. The aim of this study was to design and validate a single 14-color antibody combination for sensitive and reproducible flow cytometry monitoring of CD4+ T-cell populations in human blood to establish normal age-related reference values and evaluate the presence of potentially altered profiles in three distinct disease models-monoclonal B-cell lymphocytosis (MBL), systemic mastocytosis (SM), and common variable immunodeficiency (CVID). Overall, 145 blood samples from healthy donors were used to design and validate a 14-color antibody combination based on extensive reagent testing in multiple cycles of design-testing-evaluation-redesign, combined with
Identifiants
pubmed: 32174910
doi: 10.3389/fimmu.2020.00166
pmc: PMC7056740
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
166Informations de copyright
Copyright © 2020 Botafogo, Pérez-Andres, Jara-Acevedo, Bárcena, Grigore, Hernández-Delgado, Damasceno, Comans, Blanco, Romero, Arriba-Méndez, Gastaca-Abasolo, Pedreira, van Gaans-van den Brink, Corbiere, Mascart, van Els, Barkoff, Mayado, van Dongen, Almeida and Orfao.
Références
Cytometry A. 2019 Jun;95(6):647-654
pubmed: 30714682
Immunity. 2011 Jan 28;34(1):108-21
pubmed: 21215658
Nat Immunol. 2009 Aug;10(8):857-63
pubmed: 19578369
Clin Immunol. 2009 Jan;130(1):27-33
pubmed: 18845487
J Immunol. 2008 May 15;180(10):6713-24
pubmed: 18453591
Nat Protoc. 2019 Jul;14(7):1946-1969
pubmed: 31160786
PLoS One. 2015 Sep 14;10(9):e0138042
pubmed: 26367374
Proc Natl Acad Sci U S A. 2005 May 31;102(22):7916-21
pubmed: 15905333
J Immunol. 2018 Jan 1;200(1):336-346
pubmed: 29180490
Sci Rep. 2016 Feb 10;6:20686
pubmed: 26861911
Front Oncol. 2019 Oct 04;9:1002
pubmed: 31637216
Semin Immunol. 2013 Nov 15;25(4):252-62
pubmed: 24183700
Biomed Res Int. 2015;2015:521957
pubmed: 26583116
Alcohol Clin Exp Res. 2013 Aug;37(8):1361-9
pubmed: 23550693
Transplant Res. 2013 Oct 25;2(1):17
pubmed: 24160259
Methods Mol Biol. 2018;1745:67-82
pubmed: 29476463
J Allergy Clin Immunol. 2018 Jun;141(6):2048-2060.e13
pubmed: 28939412
Cytometry A. 2015 Apr;87(4):289-92
pubmed: 25407958
PLoS One. 2016 Feb 10;11(2):e0148260
pubmed: 26863434
Br J Haematol. 2017 Jul;178(2):257-266
pubmed: 28419441
J Allergy Clin Immunol. 2018 Jun;141(6):2208-2219.e16
pubmed: 29505809
Nature. 1999 Oct 14;401(6754):708-12
pubmed: 10537110
J Immunol. 2011 May 15;186(10):5556-68
pubmed: 21471443
J Clin Immunol. 2013 Aug;33(6):1126-33
pubmed: 23793781
Nat Immunol. 2019 Mar;20(3):301-312
pubmed: 30664737
J Immunol Methods. 2019 Dec;475:112631
pubmed: 31306640
Clin Exp Allergy. 2008 Dec;38(12):1847-57
pubmed: 19037961
Int Rev Cell Mol Biol. 2018;341:63-124
pubmed: 30262035
Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7225-30
pubmed: 26015572
J Exp Med. 1987 Nov 1;166(5):1229-44
pubmed: 2960769
Leukemia. 2017 Oct;31(10):2094-2103
pubmed: 28104919
Front Immunol. 2019 Mar 04;10:246
pubmed: 30886612
J Immunol. 2012 Apr 15;188(8):3734-44
pubmed: 22427637
Front Immunol. 2014 May 14;5:217
pubmed: 24860576
Leukemia. 2010 Nov;24(11):1927-33
pubmed: 20844562
J Immunol. 1986 Apr 1;136(7):2348-57
pubmed: 2419430
Nat Rev Immunol. 2008 Apr;8(4):247-58
pubmed: 18323851
Lab Invest. 2004 Oct;84(10):1387-98
pubmed: 15311213
Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4793-8
pubmed: 19273860
Leukemia. 2012 Sep;26(9):1986-2010
pubmed: 22948490
Cytometry A. 2008 Nov;73(11):975-83
pubmed: 18785267
Vaccine. 2013 Dec 17;32(1):111-8
pubmed: 24176499
J Viral Hepat. 2011 Jul;18(7):e341-9
pubmed: 21692946
Front Immunol. 2019 May 09;10:940
pubmed: 31143175
Annu Rev Immunol. 2003;21:425-56
pubmed: 12615888
Immunology. 2019 May;157(1):3-12
pubmed: 30897205
Eur J Immunol. 2017 Apr;47(4):607-614
pubmed: 28295238
Eur J Immunol. 2012 Sep;42(9):2215-20
pubmed: 22949319
Blood. 2009 Jan 22;113(4):769-74
pubmed: 18583570
Cytometry A. 2015 Dec;87(12):1067-9
pubmed: 26506224
Blood. 2007 May 15;109(10):4343-50
pubmed: 17234745
Am J Transplant. 2010 Nov;10(11):2410-20
pubmed: 20977632
Nat Med. 2011 Sep 18;17(10):1290-7
pubmed: 21926977
Cytometry B Clin Cytom. 2013 Jan-Feb;84(1):50-4
pubmed: 23019018
Front Immunol. 2019 May 16;10:1034
pubmed: 31156625
Cell Rep. 2015 Jun 23;11(11):1822-33
pubmed: 26074076
Eur J Immunol. 2013 Nov;43(11):2797-809
pubmed: 24258910
Lancet Infect Dis. 2019 May;19(5):e179-e186
pubmed: 30503084
Cytometry A. 2013 May;83(5):439-40
pubmed: 23450796
Immunity. 2016 May 17;44(5):1227-39
pubmed: 27178470
Cell. 2015 Apr 9;161(2):387-403
pubmed: 25772697
BMC Genomics. 2019 Jul 11;20(1):571
pubmed: 31296163
J Immunol Methods. 2019 Dec;475:112618
pubmed: 31181212
Cytometry A. 2014 Jan;85(1):25-35
pubmed: 24124072
Blood. 2012 May 10;119(19):4430-40
pubmed: 22438251
Ann N Y Acad Sci. 2013 May;1284:66-70
pubmed: 23651196
J Allergy Clin Immunol. 2018 Sep;142(3):970-973.e8
pubmed: 29746882
Nat Rev Immunol. 2018 Oct;18(10):635-647
pubmed: 30057419
J Clin Invest. 2009 Dec;119(12):3573-85
pubmed: 19920355