MicroCT optimisation for imaging fascicular anatomy in peripheral nerves.
atomy
autonomic nerves
computed tomography
microCT
neuroan
neuroanatomical mapping
peripheral nerves
Journal
Journal of neuroscience methods
ISSN: 1872-678X
Titre abrégé: J Neurosci Methods
Pays: Netherlands
ID NLM: 7905558
Informations de publication
Date de publication:
15 05 2020
15 05 2020
Historique:
received:
05
11
2019
revised:
24
02
2020
accepted:
25
02
2020
pubmed:
18
3
2020
medline:
27
5
2021
entrez:
18
3
2020
Statut:
ppublish
Résumé
Due to the lack of understanding of the fascicular organisation, vagus nerve stimulation (VNS) leads to unwanted off-target effects. Micro-computed tomography (microCT) can be used to trace fascicles from periphery and image fascicular anatomy. In this study, we present a simple and reproducible method for imaging fascicles in peripheral nerves with iodine staining and microCT for the determination of fascicular anatomy and organisation. At the determined optimal pre-processing steps and scanning parameters, the microCT protocol allowed for segmentation and tracking of fascicles within the nerves. This was achieved after 24 hours and 120 hours of staining with Lugol's solution (1% total iodine) for rat sciatic and pig vagus nerves, respectively, and the following scanning parameters: 4 μm voxel size, 35 kVp energy, 114 μA current, 4 W power, 0.25 fps in 4 s exposure time, 3176 projections and a molybdenum target. This optimised method for imaging fascicles provides high-resolution, three-dimensional images and full imaging penetration depth not obtainable with methods typically used such as histology, magnetic resonance imaging and optical coherence tomography whilst obviating time-consuming pre-processing methods, the amount of memory required, destruction of the samples and the cost associated with current microCT methods. The optimised microCT protocol facilitates segmentation and tracking of the fascicles within the nerve. The resulting segmentation map of the functional anatomical organisation of the vagus nerve will enable selective VNS ultimately allowing for the avoidance of the off-target effects and improving its therapeutic efficacy.
Sections du résumé
BACKGROUND
Due to the lack of understanding of the fascicular organisation, vagus nerve stimulation (VNS) leads to unwanted off-target effects. Micro-computed tomography (microCT) can be used to trace fascicles from periphery and image fascicular anatomy.
NEW METHOD
In this study, we present a simple and reproducible method for imaging fascicles in peripheral nerves with iodine staining and microCT for the determination of fascicular anatomy and organisation.
RESULTS
At the determined optimal pre-processing steps and scanning parameters, the microCT protocol allowed for segmentation and tracking of fascicles within the nerves. This was achieved after 24 hours and 120 hours of staining with Lugol's solution (1% total iodine) for rat sciatic and pig vagus nerves, respectively, and the following scanning parameters: 4 μm voxel size, 35 kVp energy, 114 μA current, 4 W power, 0.25 fps in 4 s exposure time, 3176 projections and a molybdenum target.
COMPARISON WITH EXISTING METHOD(S)
This optimised method for imaging fascicles provides high-resolution, three-dimensional images and full imaging penetration depth not obtainable with methods typically used such as histology, magnetic resonance imaging and optical coherence tomography whilst obviating time-consuming pre-processing methods, the amount of memory required, destruction of the samples and the cost associated with current microCT methods.
CONCLUSION
The optimised microCT protocol facilitates segmentation and tracking of the fascicles within the nerve. The resulting segmentation map of the functional anatomical organisation of the vagus nerve will enable selective VNS ultimately allowing for the avoidance of the off-target effects and improving its therapeutic efficacy.
Identifiants
pubmed: 32179090
pii: S0165-0270(20)30074-1
doi: 10.1016/j.jneumeth.2020.108652
pmc: PMC7181190
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108652Subventions
Organisme : Medical Research Council
ID : MR/R01213X/1
Pays : United Kingdom
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
Références
Prog Biophys Mol Biol. 1996;65(1-2):133-69
pubmed: 9029944
Contrast Media Mol Imaging. 2019 Feb 28;2019:8617406
pubmed: 30944550
ScientificWorldJournal. 2014 Feb 06;2014:310283
pubmed: 24683328
J Neural Eng. 2016 Aug;13(4):041002
pubmed: 27351347
Parasitology. 2018 Jun;145(7):848-854
pubmed: 29179788
Cardiol J. 2010;17(6):638-44
pubmed: 21154273
Int J Colorectal Dis. 2016 Feb;31(2):211-5
pubmed: 26546440
Front Psychiatry. 2018 Mar 13;9:44
pubmed: 29593576
J Cell Sci. 2016 Jul 1;129(13):2483-92
pubmed: 27278017
PLoS One. 2015 Jun 01;10(6):e0126230
pubmed: 26030902
Microsc Res Tech. 2004 Jun 15;64(3):250-4
pubmed: 15452892
J Neurosci Methods. 2008 Jun 30;171(2):207-13
pubmed: 18462802
Seizure. 2000 Apr;9(3):161-9
pubmed: 10775511
Gastroenterology. 2017 Mar;152(4):730-744
pubmed: 27988382
J Hand Surg Eur Vol. 2012 Jul;37(6):550-4
pubmed: 22190565
J Oral Maxillofac Pathol. 2014 Sep;18(Suppl 1):S111-6
pubmed: 25364159
J Biomed Opt. 2012 May;17(5):056012
pubmed: 22612135
Muscles Ligaments Tendons J. 2014 Jul 14;4(2):238-44
pubmed: 25332942
Indian Heart J. 2017 Sep - Oct;69(5):685-686
pubmed: 29054204
Int J Clin Pharm. 2013 Oct;35(5):744-52
pubmed: 23722455
Sci Rep. 2016 Aug 01;6:30694
pubmed: 27476584
Methods Mol Biol. 2008;455:273-92
pubmed: 18463825
Front Neurosci. 2018 Feb 07;12:49
pubmed: 29467611
PLoS Genet. 2006 Apr;2(4):e61
pubmed: 16683035
J Exp Zool B Mol Dev Evol. 2014 May;322(3):166-76
pubmed: 24482316
Heart Fail Rev. 2011 Mar;16(2):195-203
pubmed: 21165697
J Anat. 2016 Jun;228(6):889-909
pubmed: 26970556
Med Phys. 2006 Nov;33(11):4249-57
pubmed: 17153403
Clin Orthop Relat Res. 1982 Mar;(163):57-64
pubmed: 7039918
Open Access Maced J Med Sci. 2017 May 07;5(3):391-394
pubmed: 28698761
Contrast Media Mol Imaging. 2019 Mar 27;2019:7483745
pubmed: 31049044
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Dis Model Mech. 2009 May-Jun;2(5-6):206-10
pubmed: 19407324
Acta Neurol Scand. 2016 Mar;133(3):173-82
pubmed: 26190515
Biomed Res Int. 2014;2014:698256
pubmed: 25276813
J Neurosci Methods. 2019 Sep 1;325:108325
pubmed: 31260728
Am J Pharm Educ. 2007 Aug 15;71(4):78
pubmed: 17786266
Pathobiology. 2016;83(2-3):140-7
pubmed: 27100885
J Microsc. 2013 Apr;250(1):21-31
pubmed: 23432572
Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):8284-9
pubmed: 27382171
Semin Vet Med Surg (Small Anim). 1990 Feb;5(1):4-11
pubmed: 2191393
J Mater Sci Mater Med. 2015 Aug;26(8):226
pubmed: 26296419
Nature. 2013 Apr 11;496(7444):159-61
pubmed: 23579662
BMC Physiol. 2009 Jun 22;9:11
pubmed: 19545439
J Neurosci Methods. 2017 Aug 1;287:58-67
pubmed: 28634148
Gigascience. 2017 Jun 1;6(6):1-11
pubmed: 28419369
J Vis Exp. 2011 Jun 21;(52):
pubmed: 21712803
Nat Commun. 2016 Jul 04;7:12142
pubmed: 27374071
J Synchrotron Radiat. 2007 May;14(Pt 3):282-7
pubmed: 17435304
J Morphol. 2007 May;268(5):401-13
pubmed: 17372915
Circ Cardiovasc Imaging. 2010 May;3(3):314-22
pubmed: 20190279
Front Psychiatry. 2015 Mar 24;6:43
pubmed: 25852579
Cardiovasc Res. 2017 Sep 1;113(11):1270-1272
pubmed: 28859301
J Biomech. 2011 Jan 4;44(1):189-92
pubmed: 20846653
Sci Rep. 2016 Dec 21;6:39380
pubmed: 28000717
Stem Cell Res Ther. 2014 Dec 29;5(6):144
pubmed: 25689288