Brain-derived neurotrophic factor is a full endothelium-derived factor in rats.
Animals
Aorta, Abdominal
/ metabolism
Autocrine Communication
Brain-Derived Neurotrophic Factor
/ genetics
Endothelial Cells
/ metabolism
Male
Nitric Oxide
/ metabolism
Nitric Oxide Synthase Type III
/ genetics
Phosphorylation
Physical Exertion
Rats, Wistar
Receptor, trkB
/ metabolism
Sedentary Behavior
Signal Transduction
Vasodilation
Venae Cavae
/ metabolism
Artery
BDNF
Endothelium
Exercise
Vein
Journal
Vascular pharmacology
ISSN: 1879-3649
Titre abrégé: Vascul Pharmacol
Pays: United States
ID NLM: 101130615
Informations de publication
Date de publication:
Historique:
received:
12
10
2019
revised:
07
02
2020
accepted:
12
03
2020
pubmed:
18
3
2020
medline:
21
10
2020
entrez:
18
3
2020
Statut:
ppublish
Résumé
Most of what is known on vascular brain-derived neurotrophic factor (BDNF) derived from experiments on cultured endothelial cells. Therefore, the present study compared BDNF levels/localization in artery (aorta) vs vein (vena cava) from a same territory in rats either sedentary (SED) or exposed to treadmill exercise (EX) as a mean to stimulate endogenous endothelial nitric oxide (NO) production. In SED rats, for both artery and vein, BDNF was strongly expressed by endothelial cells, while only a faint and scattered expression was observed throughout the media. Endothelial and muscular BDNF staining as vascular BDNF protein levels were however higher in artery than in vein, while BDNF mRNA levels did not differ between vessels. Irrespective of the vessels, EX resulted in an increase (+50%) in BDNF protein levels with no change in BDNF mRNA levels, a selective endothelial BDNF overexpression (x4) and an increase in vascular levels of tropomyosin related kinase B receptors (TrkB) phosphorylated at tyrosine 816 (p-TrkB
Identifiants
pubmed: 32179157
pii: S1537-1891(19)30284-8
doi: 10.1016/j.vph.2020.106674
pii:
doi:
Substances chimiques
Bdnf protein, rat
0
Brain-Derived Neurotrophic Factor
0
Nitric Oxide
31C4KY9ESH
Nitric Oxide Synthase Type III
EC 1.14.13.39
Nos3 protein, rat
EC 1.14.13.39
Ntrk2 protein, rat
EC 2.7.10.1
Receptor, trkB
EC 2.7.10.1
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106674Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Disclosures No conflicts of interest, financial or otherwise, are declared by the authors. Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.