Proteogenomic analysis of granulocyte macrophage colony- stimulating factor autoantibodies in the blood of a patient with autoimmune pulmonary alveolar proteinosis.
Autoantibodies
/ blood
Autoantigens
/ immunology
Autoimmunity
Chromatography, Liquid
Disease Susceptibility
Granulocyte-Macrophage Colony-Stimulating Factor
/ immunology
Humans
Proteogenomics
/ methods
Pulmonary Alveolar Proteinosis
/ blood
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 03 2020
18 03 2020
Historique:
received:
17
09
2019
accepted:
02
03
2020
entrez:
20
3
2020
pubmed:
20
3
2020
medline:
24
11
2020
Statut:
epublish
Résumé
Recently, attempts to reveal the structures of autoantibodies comprehensively using improved proteogenomics technology, have become popular. This technology identifies peptides in highly purified antibodies by using an Orbitrap device to compare spectra from liquid chromatography-tandem mass spectrometry against a cDNA database obtained through next-generation sequencing. In this study, we first analyzed granulocyte-macrophage colony-stimulating factor (GM-CSF) autoantibodies in a patient with autoimmune pulmonary alveolar proteinosis, using the trapped ion mobility spectrometry coupled with quadrupole time-of-flight (TIMS-TOF) instrument. The TIMS-TOF instrument identified peptides that partially matched sequences in up to 156 out of 162 cDNA clones. Complementarity-determining region 3 (CDR3) was fully and partially detected in nine and 132 clones, respectively. Moreover, we confirmed one unique framework region 4 (FR4) and at least three unique across CDR3 to FR4 peptides via de novo peptide sequencing. This new technology may thus permit the comprehensive identification of autoantibody structure.
Identifiants
pubmed: 32188922
doi: 10.1038/s41598-020-61934-y
pii: 10.1038/s41598-020-61934-y
pmc: PMC7080758
doi:
Substances chimiques
Autoantibodies
0
Autoantigens
0
Granulocyte-Macrophage Colony-Stimulating Factor
83869-56-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4923Références
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