High-polyphenol extracts from Sorghum bicolor attenuate replication of Legionella pneumophila within RAW 264.7 macrophages.
Legionella
RAW 264.7
Sorghum polyphenol
macrophages
pneumophila
Journal
FEMS microbiology letters
ISSN: 1574-6968
Titre abrégé: FEMS Microbiol Lett
Pays: England
ID NLM: 7705721
Informations de publication
Date de publication:
01 04 2020
01 04 2020
Historique:
received:
19
12
2019
accepted:
17
03
2020
pubmed:
20
3
2020
medline:
25
9
2021
entrez:
20
3
2020
Statut:
ppublish
Résumé
Polyphenols derived from a variety of plants have demonstrated antimicrobial activity against diverse microbial pathogens. Legionella pneumophila is an intracellular bacterial pathogen that opportunistically causes a severe inflammatory pneumonia in humans, called Legionnaires' Disease, via replication within macrophages. Previous studies demonstrated that tea polyphenols attenuate L. pneumophila intracellular replication within mouse macrophages via increased tumor necrosis factor (TNF) production. Sorghum bicolor is a sustainable cereal crop that thrives in arid environments and is well-suited to continued production in warming climates. Sorghum polyphenols have anticancer and antioxidant properties, but their antimicrobial activity has not been evaluated. Here, we investigated the impact of sorghum polyphenols on L. pneumophila intracellular replication within RAW 264.7 mouse macrophages. Sorghum high-polyphenol extract (HPE) attenuated L. pneumophila intracellular replication in a dose-dependent manner but did not impair either bacterial replication in rich media or macrophage viability. Moreover, HPE treatment enhanced both TNF and IL-6 secretion from L. pneumophila infected macrophages. Thus, polyphenols derived from sorghum enhance macrophage restriction of L. pneumophila, likely via increased pro-inflammatory cytokine production. This work reveals commonalities between plant polyphenol-mediated antimicrobial activity and provides a foundation for future evaluation of sorghum as an antimicrobial agent.
Identifiants
pubmed: 32188994
pii: 5809964
doi: 10.1093/femsle/fnaa053
pmc: PMC8023677
pii:
doi:
Substances chimiques
Plant Extracts
0
Polyphenols
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103418
Pays : United States
Informations de copyright
© FEMS 2020.
Références
J Pharm Pharmacol. 2002 Nov;54(11):1535-44
pubmed: 12495557
J Bacteriol. 2019 Jun 21;201(14):
pubmed: 31036725
Biochem Pharmacol. 2006 Mar 14;71(6):735-41
pubmed: 16414027
J Exp Med. 2006 Apr 17;203(4):1093-104
pubmed: 16606669
Front Microbiol. 2014 Aug 20;5:434
pubmed: 25191312
J Immunol Res. 2018 Apr 12;2018:1264074
pubmed: 29850614
J Clin Microbiol. 1979 Oct;10(4):437-41
pubmed: 393713
PLoS Pathog. 2006 Mar;2(3):e18
pubmed: 16552444
Cell. 1978 Sep;15(1):261-7
pubmed: 212198
Methods Mol Biol. 2011;731:237-45
pubmed: 21516412
Proc Natl Acad Sci U S A. 2017 Nov 28;114(48):E10446-E10454
pubmed: 29133401
Infect Immun. 2001 Jun;69(6):3947-53
pubmed: 11349063
J Med Microbiol. 2002 Mar;51(3):225-230
pubmed: 11871617
Food Nutr Res. 2019 Apr 23;63:
pubmed: 31073284
FEBS Lett. 2003 May 22;543(1-3):184-9
pubmed: 12753930
Nat Rev Microbiol. 2009 Jan;7(1):13-24
pubmed: 19011659
Phytochemistry. 2004 May;65(9):1199-221
pubmed: 15184005
Curr Opin Microbiol. 2016 Feb;29:74-80
pubmed: 26709975
Nutrients. 2017 Mar 27;9(4):
pubmed: 28346392
Curr Opin Biotechnol. 2012 Apr;23(2):174-81
pubmed: 21925860
Exp Biol Med (Maywood). 2005 Oct;230(9):645-51
pubmed: 16179732
J Med Microbiol. 2019 Oct;68(10):1552-1559
pubmed: 31419210
PLoS One. 2012;7(7):e40531
pubmed: 22792362
Antimicrob Agents Chemother. 2006 Jul;50(7):2550-2
pubmed: 16801443
J Clin Microbiol. 1981 Dec;14(6):623-7
pubmed: 7037831
mBio. 2019 Nov 12;10(6):
pubmed: 31719183
Cell Microbiol. 2007 Oct;9(10):2344-57
pubmed: 17506816
Front Microbiol. 2019 May 15;10:911
pubmed: 31156565
J Agric Food Chem. 2012 Sep 12;60(36):8773-5
pubmed: 22578138
Front Microbiol. 2019 Apr 18;10:829
pubmed: 31057527
J Infect Dis. 1997 Oct;176(4):1019-28
pubmed: 9333161
Infect Immun. 1995 Sep;63(9):3253-8
pubmed: 7642253
J Med Food. 2018 Oct;21(10):990-998
pubmed: 29733262
Heliyon. 2019 May 07;5(5):e01589
pubmed: 31111105
Comp Immunol Microbiol Infect Dis. 2010 Sep;33(5):443-54
pubmed: 19732955
FEMS Microbiol Ecol. 2015 Mar;91(3):
pubmed: 25764457