Bisubstrate-Type Chemical Probes Identify GRP94 as a Potential Target of Cytosine-Containing Adenosine Analogs.


Journal

ACS chemical biology
ISSN: 1554-8937
Titre abrégé: ACS Chem Biol
Pays: United States
ID NLM: 101282906

Informations de publication

Date de publication:
17 04 2020
Historique:
pubmed: 20 3 2020
medline: 23 1 2021
entrez: 20 3 2020
Statut: ppublish

Résumé

We synthesized affinity-based chemical probes of cytosine-adenosine bisubstrate analogs and identified several potential targets by proteomic analysis. The validation of the proteomic analysis identified the chemical probe as a specific inhibitor of glucose-regulated protein 94 (GRP94), a potential drug target for several types of cancers. Therefore, as a result of the use of bisubstrate-type chemical probes and a chemical-biology methodology, this work opens the way to the development of a new family of GRP94 inhibitors that could potentially be of therapeutic interest.

Identifiants

pubmed: 32191434
doi: 10.1021/acschembio.9b00965
pmc: PMC7336334
mid: NIHMS1593591
doi:

Substances chimiques

Affinity Labels 0
Membrane Glycoproteins 0
Proteome 0
endoplasmin 0
Cytosine 8J337D1HZY
Adenosine K72T3FS567

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

952-961

Subventions

Organisme : NCI NIH HHS
ID : P01 CA186866
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States

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Auteurs

Dany Pechalrieu (D)

ETaC, CNRS FRE3600, Centre de Recherche et Développement Pierre Fabre, Toulouse, France.

Fanny Assemat (F)

ETaC, CNRS FRE3600, Centre de Recherche et Développement Pierre Fabre, Toulouse, France.

Ludovic Halby (L)

ETaC, CNRS FRE3600, Centre de Recherche et Développement Pierre Fabre, Toulouse, France.
EpiCBio, Epigenetic Chemical Biology, Department Structural Biology and Chemistry, Institut Pasteur, CNRS UMR no. 3523, 28 rue du Dr Roux, 75015 Paris, France.

Marlene Marcellin (M)

Institut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France.

Pengrong Yan (P)

Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, New York, United States.

Karima Chaoui (K)

Institut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France.

Sahil Sharma (S)

Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, New York, United States.

Gabriela Chiosis (G)

Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, New York, United States.

Odile Burlet-Schiltz (O)

Institut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France.

Paola B Arimondo (PB)

ETaC, CNRS FRE3600, Centre de Recherche et Développement Pierre Fabre, Toulouse, France.
EpiCBio, Epigenetic Chemical Biology, Department Structural Biology and Chemistry, Institut Pasteur, CNRS UMR no. 3523, 28 rue du Dr Roux, 75015 Paris, France.

Marie Lopez (M)

ETaC, CNRS FRE3600, Centre de Recherche et Développement Pierre Fabre, Toulouse, France.
Institut des Biomolécules Max Mousseron (IBMM), CNRS, Univ Montpellier, ENSCM UMR 5247, 240 Avenue du Prof. E. Jeanbrau, 34296 Montpellier Cedex 5, France.

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Classifications MeSH