Golgi-derived PI
1-Phosphatidylinositol 4-Kinase
/ genetics
ADP-Ribosylation Factor 1
/ genetics
Animals
COS Cells
Cell Line
Chlorocebus aethiops
Dynamins
/ metabolism
Endoplasmic Reticulum
/ metabolism
HeLa Cells
Humans
Membrane Microdomains
Mitochondria
/ metabolism
Mitochondrial Dynamics
Mitochondrial Membranes
/ metabolism
Phosphatidylinositol Phosphates
/ metabolism
RNA Interference
trans-Golgi Network
/ metabolism
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
20 03 2020
20 03 2020
Historique:
received:
09
04
2019
revised:
09
12
2019
accepted:
27
02
2020
entrez:
21
3
2020
pubmed:
21
3
2020
medline:
25
3
2020
Statut:
ppublish
Résumé
Mitochondrial plasticity is a key regulator of cell fate decisions. Mitochondrial division involves Dynamin-related protein-1 (Drp1) oligomerization, which constricts membranes at endoplasmic reticulum (ER) contact sites. The mechanisms driving the final steps of mitochondrial division are still unclear. Here, we found that microdomains of phosphatidylinositol 4-phosphate [PI(4)P] on trans-Golgi network (TGN) vesicles were recruited to mitochondria-ER contact sites and could drive mitochondrial division downstream of Drp1. The loss of the small guanosine triphosphatase ADP-ribosylation factor 1 (Arf1) or its effector, phosphatidylinositol 4-kinase IIIβ [PI(4)KIIIβ], in different mammalian cell lines prevented PI(4)P generation and led to a hyperfused and branched mitochondrial network marked with extended mitochondrial constriction sites. Thus, recruitment of TGN-PI(4)P-containing vesicles at mitochondria-ER contact sites may trigger final events leading to mitochondrial scission.
Identifiants
pubmed: 32193326
pii: 367/6484/1366
doi: 10.1126/science.aax6089
doi:
Substances chimiques
Phosphatidylinositol Phosphates
0
phosphatidylinositol 4-phosphate
0
1-Phosphatidylinositol 4-Kinase
EC 2.7.1.67
ADP-Ribosylation Factor 1
EC 3.6.5.2
ARF1 protein, human
EC 3.6.5.2
DNM1L protein, human
EC 3.6.5.5
Dynamins
EC 3.6.5.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1366-1371Subventions
Organisme : Medical Research Council
ID : MC_UU_00015/7
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.