Enhanced production of Dopa-incorporated mussel adhesive protein using engineered translational machineries.
3,4-dihydroxy-l-phenylalanine
mussel adhesive protein
residue-specific incorporation
tyrosyl-tRNA synthetase
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
26
12
2019
revised:
16
03
2020
accepted:
18
03
2020
pubmed:
21
3
2020
medline:
17
8
2021
entrez:
21
3
2020
Statut:
ppublish
Résumé
Mussel adhesive proteins (MAPs) have great potential as bioglues, particularly in wet conditions. Although in vivo residue-specific incorporation of 3,4-dihydroxyphenylalanine (Dopa) in tyrosine-auxotrophic Escherichia coli cells allows for production of Dopa-incorporated bioengineered MAPs (dMAPs), the low production yield hinders the practical application of dMAPs. This low production yield of dMAPs is due to low translational activity of a noncanonical amino acid, Dopa, in E. coli cells. Herein, to enhance the production yield of dMAPs, we investigated the coexpression of Dopa-recognizing tyrosyl-tRNA synthetases (TyrRSs). To use the Dopa-specific Methanococcus jannaschii TyrRS (MjTyrRS-Dopa), we altered the anticodon of tyrosyl-tRNA amber suppressor into AUA (MjtRNA
Substances chimiques
Codon
0
Proteins
0
adhesive protein, mussel
0
Dihydroxyphenylalanine
63-84-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1961-1969Subventions
Organisme : Ministry of Science and ICT, National Research Foundation of Korea
ID : 2018R1A2B3003758
Pays : International
Organisme : Ministry of Science and ICT, National Research Foundation of Korea
ID : 2019R1A2C108491011
Pays : International
Organisme : Marine Biotechnology Program (Marine BioMaterials Research Center)
Pays : International
Informations de copyright
© 2020 Wiley Periodicals, Inc.
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