Nov/CCN3 Enhances Cord Blood Engraftment by Rapidly Recruiting Latent Human Stem Cell Activity.
C-MYC
HSC recruitment
NOV (CCN3)
ROS
hematopoietic stem cell
hexokinase 2
stem cell state transition
umbilical cord blood transplantation
Journal
Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472
Informations de publication
Date de publication:
02 04 2020
02 04 2020
Historique:
received:
04
04
2019
revised:
04
01
2020
accepted:
19
02
2020
pubmed:
21
3
2020
medline:
28
4
2021
entrez:
21
3
2020
Statut:
ppublish
Résumé
Umbilical cord blood (UCB) has had considerable impact in pediatric stem cell transplantation, but its wider use is limited in part by unit size. Long-term ex vivo culture offers one approach to increase engraftment capacity by seeking to expand stem and progenitor cells. Here, we show brief incubation (8 h) of UCB CD34+ cells with the matricellular regulator Nov (CCN3) increases the frequency of serially transplantable hematopoietic stem cells (HSCs) 6-fold. This rapid response suggests recruitment rather than expansion of stem cells; accordingly, in single-cell assays, Nov increases the clonogenicity of phenotypic HSCs without increasing their number through cell division. Recruitment is associated with both metabolic and transcriptional changes, and tracing of cell divisions demonstrates that the increased clonogenic activity resides within the undivided fraction of cells. Harnessing latent stem cell potential through recruitment-based approaches will inform understanding of stem cell state transitions with implications for translation to the clinic.
Identifiants
pubmed: 32197066
pii: S1934-5909(20)30065-5
doi: 10.1016/j.stem.2020.02.012
pmc: PMC7118368
pii:
doi:
Substances chimiques
Antigens, CD34
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
527-541.e8Subventions
Organisme : Medical Research Council
ID : MC_U137973817
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N000838/1
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C36048/17901
Pays : United Kingdom
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
Références
Cell. 2015 Jun 18;161(7):1553-65
pubmed: 26073944
Biol Blood Marrow Transplant. 2018 May;24(5):909-913
pubmed: 29360515
Nat Med. 2018 Mar;24(3):360-367
pubmed: 29377004
Nat Med. 2010 Feb;16(2):232-6
pubmed: 20081862
Nature. 2014 May 1;509(7498):49-54
pubmed: 24670665
J Biol Chem. 2013 May 3;288(18):12654-66
pubmed: 23530034
Cell Stem Cell. 2015 Mar 5;16(3):302-13
pubmed: 25704240
Biochem Biophys Res Commun. 2010 Apr 16;394(4):1075-81
pubmed: 20346347
Blood. 2010 May 6;115(18):3704-7
pubmed: 20207983
Cell Stem Cell. 2018 May 3;22(5):627-638
pubmed: 29727678
Science. 2007 Apr 27;316(5824):590-3
pubmed: 17463287
Transfusion. 2014 Jan;54(1):231-7
pubmed: 23718644
J Immunol Methods. 2009 Aug 15;347(1-2):70-8
pubmed: 19567251
Blood. 2001 Jan 15;97(2):435-41
pubmed: 11154220
Science. 2011 Jul 8;333(6039):218-21
pubmed: 21737740
Br J Haematol. 2016 Feb;172(3):360-70
pubmed: 26577457
Cell. 2017 May 18;169(5):807-823.e19
pubmed: 28479188
Blood. 1996 Nov 1;88(9):3306-13
pubmed: 8896394
Science. 2014 Sep 19;345(6203):1509-12
pubmed: 25237102
Cell Stem Cell. 2016 Jan 7;18(1):144-55
pubmed: 26669897
Cell Stem Cell. 2019 Nov 7;25(5):639-653.e7
pubmed: 31631013
Vox Sang. 2006 Oct;91(3):195-205
pubmed: 16958831
Int J Mol Med. 2015 Dec;36(6):1451-63
pubmed: 26498181
Blood. 2015 Mar 5;125(10):1562-5
pubmed: 25593337
Cell Stem Cell. 2018 Jun 1;22(6):792-793
pubmed: 29859169
Int J Hematol. 2014 Apr;99(4):393-406
pubmed: 24563081
Nat Rev Mol Cell Biol. 2014 Apr;15(4):243-56
pubmed: 24651542
Stem Cell Rev. 2006;2(3):171-80
pubmed: 17625253
Transfus Apher Sci. 2016 Jun;54(3):364-72
pubmed: 27211041
Cell Stem Cell. 2012 Feb 3;10(2):120-36
pubmed: 22305562
Science. 2010 Sep 10;329(5997):1345-8
pubmed: 20688981
Cell Stem Cell. 2016 Jan 7;18(1):10-2
pubmed: 26748750
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9836-41
pubmed: 9275212
J Endocrinol. 2003 Aug;178(2):169-75
pubmed: 12904165
Cell Stem Cell. 2007 Dec 13;1(6):635-45
pubmed: 18371405
Development. 2014 Dec;141(24):4656-66
pubmed: 25468935
Nat Cell Biol. 2018 Jun;20(6):710-720
pubmed: 29802403
J Cell Biochem. 2007 Aug 15;101(6):1475-91
pubmed: 17340618
Stem Cells Transl Med. 2017 May;6(5):1312-1315
pubmed: 28456009
Blood. 2015 May 7;125(19):2885-92
pubmed: 25778529
Stem Cells. 2010 Sep;28(9):1571-80
pubmed: 20641035
Immunity. 1994 Nov;1(8):661-73
pubmed: 7541305
Nat Rev Mol Cell Biol. 2013 Jun;14(6):329-40
pubmed: 23698583
Cell Stem Cell. 2011 Oct 4;9(4):298-310
pubmed: 21982230
J Biol Chem. 2009 Oct 23;284(43):29935-44
pubmed: 19706598