The unprecedented membrane deformation of the human nuclear envelope, in a magnetic field, indicates formation of nuclear membrane invaginations.
Cell Membrane
/ metabolism
Cholesterol
/ metabolism
Fatty Acids, Unsaturated
/ metabolism
Humans
Kidney
/ pathology
Magnetic Fields
Magnetic Resonance Spectroscopy
Membrane Fluidity
/ physiology
Membrane Lipids
/ metabolism
Nuclear Envelope
/ metabolism
Phase Transition
Phosphatidylinositols
/ metabolism
Phospholipids
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 03 2020
20 03 2020
Historique:
received:
06
11
2019
accepted:
14
01
2020
entrez:
22
3
2020
pubmed:
22
3
2020
medline:
7
1
2021
Statut:
epublish
Résumé
Human nuclear membrane (hNM) invaginations are thought to be crucial in fusion, fission and remodeling of cells and present in many human diseases. There is however little knowledge, if any, about their lipid composition and dynamics. We therefore isolated nuclear envelope lipids from human kidney cells, analyzed their composition and determined the membrane dynamics after resuspension in buffer. The hNM lipid extract was composed of a complex mixture of phospholipids, with high amounts of phosphatidylcholines, phosphatidylinositols (PI) and cholesterol. hNM dynamics was determined by solid-state NMR and revealed that the lamellar gel-to-fluid phase transition occurs below 0 °C, reflecting the presence of elevated amounts of unsaturated fatty acid chains. Fluidity was higher than the plasma membrane, illustrating the dual action of Cholesterol (ordering) and PI lipids (disordering). The most striking result was the large magnetic field-induced membrane deformation allowing to determine the membrane bending elasticity, a property related to hydrodynamics of cells and organelles. Human Nuclear Lipid Membranes were at least two orders of magnitude more elastic than the classical plasma membrane suggesting a physical explanation for the formation of nuclear membrane invaginations.
Identifiants
pubmed: 32198481
doi: 10.1038/s41598-020-61746-0
pii: 10.1038/s41598-020-61746-0
pmc: PMC7083927
doi:
Substances chimiques
Fatty Acids, Unsaturated
0
Membrane Lipids
0
Phosphatidylinositols
0
Phospholipids
0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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