Common occurrence of divergent Cryptosporidium species and Cryptosporidium parvum subtypes in farmed bamboo rats (Rhizomys sinensis).
Animals
China
/ epidemiology
Cryptosporidiosis
/ epidemiology
Cryptosporidium
/ genetics
Cryptosporidium parvum
/ genetics
DNA, Protozoan
Farms
Feces
/ parasitology
Genes, rRNA
Genotype
Humans
Molecular Epidemiology
Muridae
/ parasitology
Oocysts
Polymerase Chain Reaction
/ veterinary
Zoonoses
/ diagnosis
Bamboo rat
Cryptosporidium
Molecular epidemiology
Subtype
Zoonotic
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
24 Mar 2020
24 Mar 2020
Historique:
received:
03
08
2019
accepted:
16
03
2020
entrez:
25
3
2020
pubmed:
25
3
2020
medline:
24
11
2020
Statut:
epublish
Résumé
Bamboo rats are widely farmed in southern China for meat, but their potential in transmitting pathogens to humans and other farm animals remains unclear. To understand the transmission of Cryptosporidium spp. in these animals, 709 fecal samples were collected in this study from Chinese bamboo rats (Rhizomys sinensis) on nine farms in Jiangxi, Guangxi and Hainan provinces, China. They were analyzed for Cryptosporidium spp. using PCR and sequence analyses of the small subunit rRNA gene. Cryptosporidium parvum, C. parvum-like and C. ubiquitum-like genotypes identified were subtyped by sequence analysis of the 60 kDa glycoprotein (gp60) gene. Altogether, Cryptosporidium spp. were detected in 209 (29.5%) samples. The detection rate in samples from animals under two months of age (70.0%,105/150) was significantly higher than in samples from animals above 2 months (18.6%, 104/559; χ Results of the present study suggest that bamboo rats on the study farms were infected with diverse Cryptosporidium species and divergent C. parvum subtypes, which probably had originated from their native habitats. As similar C. parvum subtypes have been recently detected in humans and farmed macaques, attentions should be paid to the potential role of these new farm animals in the transmission of zoonotic pathogens.
Sections du résumé
BACKGROUND
BACKGROUND
Bamboo rats are widely farmed in southern China for meat, but their potential in transmitting pathogens to humans and other farm animals remains unclear.
METHODS
METHODS
To understand the transmission of Cryptosporidium spp. in these animals, 709 fecal samples were collected in this study from Chinese bamboo rats (Rhizomys sinensis) on nine farms in Jiangxi, Guangxi and Hainan provinces, China. They were analyzed for Cryptosporidium spp. using PCR and sequence analyses of the small subunit rRNA gene. Cryptosporidium parvum, C. parvum-like and C. ubiquitum-like genotypes identified were subtyped by sequence analysis of the 60 kDa glycoprotein (gp60) gene.
RESULTS
RESULTS
Altogether, Cryptosporidium spp. were detected in 209 (29.5%) samples. The detection rate in samples from animals under two months of age (70.0%,105/150) was significantly higher than in samples from animals above 2 months (18.6%, 104/559; χ
CONCLUSIONS
CONCLUSIONS
Results of the present study suggest that bamboo rats on the study farms were infected with diverse Cryptosporidium species and divergent C. parvum subtypes, which probably had originated from their native habitats. As similar C. parvum subtypes have been recently detected in humans and farmed macaques, attentions should be paid to the potential role of these new farm animals in the transmission of zoonotic pathogens.
Identifiants
pubmed: 32204732
doi: 10.1186/s13071-020-04021-5
pii: 10.1186/s13071-020-04021-5
pmc: PMC7092434
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
149Subventions
Organisme : National Natural Science Foundation of China
ID : 31820103014
Organisme : National Natural Science Foundation of China
ID : 31630078
Organisme : 111 Project
ID : D20008
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