Intraventricular dopamine infusion alleviates motor symptoms in a primate model of Parkinson's disease.


Journal

Neurobiology of disease
ISSN: 1095-953X
Titre abrégé: Neurobiol Dis
Pays: United States
ID NLM: 9500169

Informations de publication

Date de publication:
06 2020
Historique:
received: 22 01 2020
revised: 24 02 2020
accepted: 18 03 2020
pubmed: 25 3 2020
medline: 13 4 2021
entrez: 25 3 2020
Statut: ppublish

Résumé

Continuous compensation of dopamine represents an ideal symptomatic treatment for Parkinson's disease (PD). The feasibility in intracerebroventricular administration (i.c.v.) of dopamine previously failed because of unresolved dopamine oxidation. We aim to test the feasibility, safety margins and efficacy of continuous i.c.v. of anaerobic-dopamine (A-dopamine) with a pilot translational study in a non-human primate model of PD. Continuous and circadian i.c.v. of A-dopamine was administered through a micro-pump connected to a subcutaneous catheter implanted into the right frontal horn of 8 non-human primates treated with 1-methyl-4- phenyl-1,2,3,6-tetrahydropyridine (MPTP). A-dopamine was assessed at acute doses previously reported for dopamine as well as evaluating the long term therapeutic index of A-dopamine in comparison to anaerobically prepared L-dopa or methyl ester L-dopa. Over 60 days of a continuous circadian i.c.v. of A-dopamine improved motor symptoms (therapeutic index from 30 to 70 mg/day) without tachyphylaxia. No dyskinesia was observed even with very high doses. Death after 1 to 10 days (without neuronal alteration) was only observed with doses in excess of 160 mg whereas L-dopa i.c.v. was not effective at any dose. The technical feasibility of the administration regimen was confirmed for an anaerobic preparation of dopamine and for administration of a minimal infusion volume by micro-pump at a constant flow that prevented obstruction. Continuous circadian i.c.v. of A-dopamine appears to be feasible and shows efficacy without dyskinesia with a safe therapeutic index.

Sections du résumé

BACKGROUND
Continuous compensation of dopamine represents an ideal symptomatic treatment for Parkinson's disease (PD). The feasibility in intracerebroventricular administration (i.c.v.) of dopamine previously failed because of unresolved dopamine oxidation.
OBJECTIVES
We aim to test the feasibility, safety margins and efficacy of continuous i.c.v. of anaerobic-dopamine (A-dopamine) with a pilot translational study in a non-human primate model of PD.
METHODS
Continuous and circadian i.c.v. of A-dopamine was administered through a micro-pump connected to a subcutaneous catheter implanted into the right frontal horn of 8 non-human primates treated with 1-methyl-4- phenyl-1,2,3,6-tetrahydropyridine (MPTP). A-dopamine was assessed at acute doses previously reported for dopamine as well as evaluating the long term therapeutic index of A-dopamine in comparison to anaerobically prepared L-dopa or methyl ester L-dopa.
RESULTS
Over 60 days of a continuous circadian i.c.v. of A-dopamine improved motor symptoms (therapeutic index from 30 to 70 mg/day) without tachyphylaxia. No dyskinesia was observed even with very high doses. Death after 1 to 10 days (without neuronal alteration) was only observed with doses in excess of 160 mg whereas L-dopa i.c.v. was not effective at any dose. The technical feasibility of the administration regimen was confirmed for an anaerobic preparation of dopamine and for administration of a minimal infusion volume by micro-pump at a constant flow that prevented obstruction.
CONCLUSION
Continuous circadian i.c.v. of A-dopamine appears to be feasible and shows efficacy without dyskinesia with a safe therapeutic index.

Identifiants

pubmed: 32205254
pii: S0969-9961(20)30121-2
doi: 10.1016/j.nbd.2020.104846
pii:
doi:

Substances chimiques

Antiparkinson Agents 0
Dopamine Agonists 0
Levodopa 46627O600J
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 9P21XSP91P
levodopa methyl ester M30686U4X4
Dopamine VTD58H1Z2X

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

104846

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Caroline Moreau (C)

Expert Center for Parkinson's Disease, Univ Lille, Inserm, CHU Lille, UMR_S1171, Lille F-59000, France.

Anne Sophie Rolland (AS)

Medical Pharmacology Department, Univ Lille, Inserm, CHU Lille, UMR_S1171, Lille F-59000, France.

Elsa Pioli (E)

Motac Neuroscience Ltd, Manchester, United Kingdom.

Qin Li (Q)

Motac Neuroscience Ltd, Manchester, United Kingdom.

Pascal Odou (P)

University of Lille, GRITA EA 7365, France; Pharmacy of CHU of Lille, University of Lille, France.

Christine Barthelemy (C)

Pharmacy of CHU of Lille, University of Lille, France.

Damien Lannoy (D)

University of Lille, GRITA EA 7365, France; Pharmacy of CHU of Lille, University of Lille, France.

Alexandre Demailly (A)

InBrain Pharma, Lille, France.

Natacha Carta (N)

Pharmacy of CHU of Lille, University of Lille, France.

Vincent Deramecourt (V)

Univ. Lille, INSERM UMRS 1172, CHU Lille, UMRS 1172 LabEx DISTALZ, Lille F-59000, France.

Florent Auger (F)

Medical Pharmacology Department, Univ Lille, Inserm, CHU Lille, UMR_S1171, Lille F-59000, France.

Gregory Kuchcinski (G)

Department of Neuroradiology, Univ Lille, CHU Lille, Inserm, UMR_S 1171, Lille, France.

Charlotte Laloux (C)

Medical Pharmacology Department, Univ Lille, Inserm, CHU Lille, UMR_S1171, Lille F-59000, France.

Luc Defebvre (L)

Expert Center for Parkinson's Disease, Univ Lille, Inserm, CHU Lille, UMR_S1171, Lille F-59000, France.

Regis Bordet (R)

Medical Pharmacology Department, Univ Lille, Inserm, CHU Lille, UMR_S1171, Lille F-59000, France.

James Duce (J)

The ALBORADA Drug Discovery Institute, University of Cambridge, Cambridge Biomedical Campus, Hills Road, Cambridge, UK; Melbourne Dementia Research Centre, The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, Australia.

Jean Christophe Devedjian (JC)

Medical Pharmacology Department, Univ Lille, Inserm, CHU Lille, UMR_S1171, Lille F-59000, France.

Erwan Bezard (E)

Motac Neuroscience Ltd, Manchester, United Kingdom; Université de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293, Bordeaux F-33000, France; CNRS, Institut des Maladies Neurodégénératives, UMR 5293, Bordeaux F-33000, France.

Matthieu Fisichella (M)

InBrain Pharma, Lille, France.

David Devos (D)

Medical Pharmacology Department, Univ Lille, Inserm, CHU Lille, UMR_S1171, Lille F-59000, France. Electronic address: david.devos@chru-lille.fr.

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Classifications MeSH