Bioprinting for Human Respiratory and Gastrointestinal In Vitro Models.
A549 Cells
Alveolar Epithelial Cells
Automation
Biocompatible Materials
Bioprinting
/ methods
Bronchi
/ cytology
Caco-2 Cells
Drug Evaluation, Preclinical
Electric Impedance
Epithelial Cells
Equipment Design
Gastrointestinal Tract
/ cytology
Humans
In Vitro Techniques
Intestinal Mucosa
/ cytology
L-Lactate Dehydrogenase
/ analysis
Microscopy, Confocal
Microscopy, Fluorescence
Printing, Three-Dimensional
Tissue Engineering
/ methods
Toxicity Tests
Alveolar epithelial cells
Bioprinting technique
Bronchial epithelial cells
In vitro cultures
Intestine epithelial cells
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
25
3
2020
pubmed:
25
3
2020
medline:
9
3
2021
Statut:
ppublish
Résumé
Increasing ethical and biological concerns require a paradigm shift toward animal-free testing strategies for drug testing and hazard assessments. To this end, the application of bioprinting technology in the field of biomedicine is driving a rapid progress in tissue engineering. In particular, standardized and reproducible in vitro models produced by three-dimensional (3D) bioprinting technique represent a possible alternative to animal models, enabling in vitro studies relevant to in vivo conditions. The innovative approach of 3D bioprinting allows a spatially controlled deposition of cells and biomaterial in a layer-by-layer fashion providing a platform for engineering reproducible models. However, despite the promising and revolutionizing character of 3D bioprinting technology, standardized protocols providing detailed instructions are lacking. Here, we provide a protocol for the automatized printing of simple alveolar, bronchial, and intestine epithelial cell layers as the basis for more complex respiratory and gastrointestinal tissue models. Such systems will be useful for high-throughput toxicity screening and drug efficacy evaluation.
Identifiants
pubmed: 32207114
doi: 10.1007/978-1-0716-0520-2_13
doi:
Substances chimiques
Biocompatible Materials
0
L-Lactate Dehydrogenase
EC 1.1.1.27
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
199-215Références
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