Altered Immunity of Laboratory Mice in the Natural Environment Is Associated with Fungal Colonization.

Animals Autophagy-Related Proteins / genetics CD8-Positive T-Lymphocytes Environment Feces / microbiology Female Fungi / genetics Gastrointestinal Microbiome / immunology Granulocytes / immunology Immune System Intestines / microbiology Lymphocytes Male Mice Mice, Inbred C57BL Mice, Knockout Mycobiome / immunology Nod2 Signaling Adaptor Protein / genetics
Aspergillus fungi granulocytes laboratory mice mesocosm microbiota mycobiota neutrophils rewilding wild mice

Journal

Cell host & microbe
ISSN: 1934-6069
Titre abrégé: Cell Host Microbe
Pays: United States
ID NLM: 101302316

Informations de publication

Date de publication:
13 05 2020
Historique:
received: 01 10 2019
revised: 11 12 2019
accepted: 28 02 2020
pubmed: 27 3 2020
medline: 20 1 2021
entrez: 27 3 2020
Statut: ppublish

Résumé

Free-living mammals, such as humans and wild mice, display heightened immune activation compared with artificially maintained laboratory mice. These differences are partially attributed to microbial exposure as laboratory mice infected with pathogens exhibit immune profiles more closely resembling that of free-living animals. Here, we examine how colonization by microorganisms within the natural environment contributes to immune system maturation by releasing inbred laboratory mice into an outdoor enclosure. In addition to enhancing differentiation of T cell populations previously associated with pathogen exposure, outdoor release increased circulating granulocytes. However, these "rewilded" mice were not infected by pathogens previously implicated in immune activation. Rather, immune system changes were associated with altered microbiota composition with notable increases in intestinal fungi. Fungi isolated from rewilded mice were sufficient in increasing circulating granulocytes. These findings establish a model to investigate how the natural environment impacts immune development and show that sustained fungal exposure impacts granulocyte numbers.

Identifiants

DOI: 10.1016/j.chom.2020.02.015 PMID: 32209432 PMC: PMC7276265
pubmed: 32209432
pii: S1931-3128(20)30123-2
doi: 10.1016/j.chom.2020.02.015
pmc: PMC7276265
mid: NIHMS1574229
pii:
doi:

Substances chimiques

Atg16l1 protein, mouse 0
Autophagy-Related Proteins 0
Nod2 Signaling Adaptor Protein 0
Nod2 protein, mouse 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

809-822.e6

Subventions

Organisme : NCATS NIH HHS
ID : UL1 TR001445
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA016087
Pays : United States
Organisme : NIDDK NIH HHS
ID : F30 DK122698
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK093668
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL125816
Pays : United States
Organisme : NIH HHS
ID : S10 OD018338
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI140754
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI130945
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI121244
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI133977
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK103788
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL084312
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL123340
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI100853
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI137336
Pays : United States
Organisme : NIH HHS
ID : S10 OD010580
Pays : United States

Commentaires et corrections

Type : CommentIn
Type : CommentIn

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests K.C. receives research funding from Pfizer and Abbvie and P.L. receives research funding from Pfizer. K.C. has consulted for or received an honorarium from Puretech Health, Genentech, and Abbvie. K.C. has a provisional patent, U.S. Patent Application. No. 15/625,934. P.L. consults for and has equity in Toilabs. P.L. is a federal employee.

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Auteurs

Frank Yeung (F)

Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY 10016, USA; Sackler Institute of Graduate Biomedical Sciences, New York University Grossman School of Medicine, New York, NY 10016, USA.

Ying-Han Chen (YH)

Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY 10016, USA.

Jian-Da Lin (JD)

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Jacqueline M Leung (JM)

Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA.

Caroline McCauley (C)

Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.

Joseph C Devlin (JC)

Sackler Institute of Graduate Biomedical Sciences, New York University Grossman School of Medicine, New York, NY 10016, USA; Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.

Christina Hansen (C)

Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA.

Alex Cronkite (A)

Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY 10016, USA; Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA.

Zac Stephens (Z)

Division of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.

Charlotte Drake-Dunn (C)

Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY 10016, USA.

Yi Fulmer (Y)

Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA; Division of Infectious Disease, Department of Medicine, New York University Langone Health, New York, NY 10016, USA.

Bo Shopsin (B)

Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA; Division of Infectious Disease, Department of Medicine, New York University Langone Health, New York, NY 10016, USA.

Kelly V Ruggles (KV)

Division of Translational Medicine, Department of Medicine, New York University Langone Health, New York, NY 10016, USA; Applied Bioinformatics Laboratories, New York Unversity Grossman School of Medicine, New York, NY 10016, USA.

June L Round (JL)

Division of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.

P'ng Loke (P)

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: png.loke@nih.gov.

Andrea L Graham (AL)

Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA. Electronic address: algraham@princeton.edu.

Ken Cadwell (K)

Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine, New York, NY 10016, USA; Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016, USA; Division of Gastroenterology and Hepatology, Department of Medicine, New York University Langone Health, New York, NY 10016, USA. Electronic address: ken.cadwell@med.nyu.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines associées à l'autophagie › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › Lymphocytes T CD8+ › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Environnement et santé publique › Environnement › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Liquides et sécrétions biologiques › Fèces › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Eucaryotes › Champignons › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbiome gastro-intestinal › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Granulocytes › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Système digestif › Tube digestif › Intestins › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Souris knockout › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Mycobiome › Altered Immunity of Laboratory Mice in the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Protéines adaptatrices de signalisation CARD › Protéine adaptatrice de signalisation NOD2 › Altered Immunity of Laboratory Mice in the...