Combined (d)SPE-QuEChERS Extraction of Mycotoxins in Mixed Feed Rations and Analysis by High Performance Liquid Chromatography-High-Resolution Mass Spectrometry.
Mycotoxins
QuEChERS
data independent SWATH
dispersive solid phase extraction
high-resolution mass spectrometry
liquid chromatography
mixed feed rations
Journal
Toxins
ISSN: 2072-6651
Titre abrégé: Toxins (Basel)
Pays: Switzerland
ID NLM: 101530765
Informations de publication
Date de publication:
23 03 2020
23 03 2020
Historique:
received:
16
12
2019
revised:
12
03
2020
accepted:
13
03
2020
entrez:
27
3
2020
pubmed:
27
3
2020
medline:
6
3
2021
Statut:
epublish
Résumé
The objective of this work was the development of a methodology capable of simultaneously determine 26 mycotoxins in mixed feed rations collected in 20 dairy farms. A sample preparation methodology based on a combination of (d)SPE and QuEChERS extractions was used. Liquid chromatography-high resolution mass spectrometry was employed for both identification and quantification purposes. To this respect, a powerful workflow based on data-independent acquisition, consisting of fragmenting all precursor ions entering the mass spectrometer in narrow m/z isolation windows (SWATH), was implemented. SWATH data file then contains all the information that would be acquired in a multitude of different experimental approaches in a single all-encompassing dataset. Analytical method performance was evaluated in terms of linearity, repeatability and matrix effect. Relative recoveries were also measured, giving values above 80% for most compounds. Matrix-matched calibration was carried out and enabled reaching the low ng mL
Identifiants
pubmed: 32210164
pii: toxins12030206
doi: 10.3390/toxins12030206
pmc: PMC7150789
pii:
doi:
Substances chimiques
Mycotoxins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Axencia Galega de Innovación
ID : CONECTA-PEME GALAC project IN852A 2016/103
Pays : International
Organisme : Axencia Galega de Innovación
ID : Competitive Research Groups GPC 2017/04
Pays : International
Organisme : Axencia Galega de Innovación
ID : Competitive Research Group GPC IN607B 2019/13
Pays : International
Organisme : Ministerio de Ciencia e Innovación
ID : IGDC13-1E-2026 (Infrastructure Program)
Pays : International
Organisme : CRETUS Strategic Partnership
ID : ED431E 2018/01
Pays : International
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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